Activity Evaluation of Sulfate Reducing Bacteria for the Sulfate Removal in the Mine Drainage

doi:10.12972/ksmer.2016.53.5.387

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2016 Vol.53, Issue 5 Next Page

Research Paper (Special Issue)

Activity Evaluation of Sulfate Reducing Bacteria for the Sulfate Removal in the Mine Drainage 광산배수에서 황산염 제거를 위한 황산염환원균의 활성도 연구: 박현성·고명수·이상환·홍지혜·조상현·유지용·조정희·이종운
Hyun-Sung Park, Myoung-Soo Ko, Sang-Hwan Lee, Ji-Hye Hong, Sang-Hyun Cho, Ji-Yong Yu, Jeong-Hee Jo and Jong-Un Lee

31 October 2016. pp. 387-397

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Abstract

Several substrates were applied to evaluate the sulfate reducing bacteria(SRB) activity and sulfate removal from sulfate-rich mine drainage. Among the substrates, single cow manure and its mixture induced sulfate reduction; in addition, 90% of total sulfur was removed from mine drainage. The sulfates in Postgate medium added to these inocula were also reduced by 90% after 22 days. Although sulfide reduced by SRB existed in the form of H2S and HS‒in circumstantial pH, it was probably removed by gas dissociation of H2S because of low divalent metal concentrations inmine drainage. Inocula incubated onto Postgate media in anaerobic condition showed more heterotrophic anaerobe colony in mixture of cow manure and mushroom compost due to microbial diversity. The subsidiaries such as limestone and rice straw did not concerned SRB activity and removal of sulfur species, and it could be appled to substrate mixtures to enhance permeability and to prevent the channeling in full-scale passive treatment.

Keywords

Mine drainage

Substrate

Bioreactor

Sulfate reducing bacteria

Sulfate reduction

황산염 함량이 높은 광산배수를 대상으로 다양한 기질물질을 적용하여 황산염환원균의 활성도와 황산염 제거율을 평가하였다. 적용 기질물질 중 우분 단독 또는 우분이 포함된 버섯퇴비 및 혼합퇴비에서 황산염이 대부분 환원되고 총 황의 제거율이 90% 이상 제거되었다. 우분과 우분이 포함된 기질물질 조합을 Postgate 액상배지에 접종하여 황산염 환원 양상을 분석한 결과 22일 경과 후 90% 이상 황산염이 환원되는 것으로 나타났다. 황산염환원균에 의해 환원된 황산염은 주어진 pH 조건에서 HS‒또는 H2S로 존재할 것으로 예측되고, 광산배수 내 낮은 2가 중금속 함량으로 인해 H2S가 기체상으로 해리되어 총 황이 제거 되는 것으로 추측된다. 또한 Postgate 평판배지에 접종하여 혐기적 조건에 배양한 결과 우분과 버섯퇴비가 상대적으로 많은 군집이 계수되었는데 이는 버섯퇴비 내 존재하는 다양한 혐기적 종속영양균 때문인 것으로 보인다. 기질물질의 자연정화공정 적용 시 발생하는 투수율 저감과 편류현상 방지를 위해 보조제로 석회석과 볏짚을 첨가한 결과 황산염 환원과 용존 총 황의 제거에 영향을 미치지 않아 보조제로서 적용이 가능한 것으로 판단된다.

키워드

광산배수

기질물질

생물반응기

황산염환원균

황산염 환원

References

APHA, 1998, “Standard Methods for the Examination of Water and Wastewater, In: Clesceri, L.S., Greenberg, A.E., Eaton, A.D.(Eds.),” Washington, DC.
Be´chard, G., Yamazaki, H., Gould, W.D. and Be´dard, P., 1994, “Use of cellulosic substrates for the microbial treatment of acid mine drainage,” J. Environ. Qual. Vol. 23, pp. 111-116.
Chen, B.Y., Utgikar, V.P., Harmon, S.M., Tabak, H.H., Bishop, D.F. and Govind, R. 2000, “Studies of biosorption of zinc(II) and copper(II) on Desulfovibrio desulfuricans,” Int. Biodeterior. Biodegrad., Vol. 46, pp. 11-18.
Clark D.S., 1967, “Comparison of pour and surface plate methods for determination of bacterial count,” Canadian Journal of Microbiology, Vol. 13, No. 11, pp. 1409-1412.
David, J.R., Vijayakumar S., Souhail R. Al-Abeda, Derrick, A., 2007, “Statistical validation of sulfate quantification methods used for analysis of acid mine drainage,” Talanta, Vol. 71, No. 1, pp. 303-311.
Gazea, B., Adam, K. and Kontopoulos., A. 1996, “A review of passive systems for the treatment of acid mine drainage,” Miner. Eng., Vol. 9, pp. 23-42.
Gray, N.F. and O’'Neill, C., 1997, “Acid mine drainage toxicity testing,” Environ. Geochem. Health, Vol. 19, pp. 165-171.
Hulshof, A.H.M., Blowes, D.W., Ptacek, C.J. and Gould, W.D., 2003, “Microbial and nutrient investigations into the use of in situ layers for treatment of tailings effluent,” Environ. Sci. Technol., Vol. 37, pp. 5027-5033.
Jong, T. and Parry, D.L., 2003, “Removal of sulfate and heavy metals by sulfate reducing bacteria in short-term bench scale upflow anaerobic packed bed reactor runs,” Water Research, Vol. 37, pp. 3379-3389.
Jong, T. and Parry, D.L., 2004, “Heavy metal speciation in solid-phase materials from abacterial sulfate reducing bioreactor using sequential extraction procedure combined with acid volatile sulfide analysis,” J. Environ. Monit., Vol. 6, pp. 278-285.
Lens, P., Vallero, M., Esposito, G. and Zandvoort, M., 2002, “Perspectives of sulfate reducing bioreactors in environmental biotechnology,” Re/Views in Environmental Science and Bio/Technology, Vol. 1, pp. 311-325.
Logan, M.V., Reardon, K.F., Figueroa, L.A., McLain, J.E.T. and Ahmann, D.M., 2005, “Microbial community activities during establishment, performance, and decline of bench- scale passive treatment systems for mine drainage,” Water Research, Vol. 39, pp. 4537-4551.
Manabu, F. and Susumu, T., 1989, “Reduction of tetrazolium salts by sulfate-reducing bacteria,” FEMS Microbiology Ecology, Vol. 5, No. 1, pp. 13-19.
Perry, R.H. and Green, D., 1984, “Perry’s Chemical Engineer’s Handbook, sixth ed.,” McGraw-Hill Book Company, Singapore.
PIRAMID Consortium, 2003, “Engineering guidelines for the passive remediation of acidic and metalliferous mine drainage and similar wastewaters,” European Commission 5th Framework RTD Project EVK1-CT-1999-000021, University of Newcastle Upon Tyne, Newcastle Upon Tyne, UK.
Plumlee, G.S., Smith, K.S., Montour, M.R., Ficklin, W.H. and Mosier, E.L., 1999, “Geologic Controls on the Composition of Natural Waters and Mine Waters Draining Diverse Mineral-Deposit Types. In: L.H. Filipek and G.S. Plumlee (Eds.), The Environmental Geochemistry of Mineral Deposits, Part B: Case Studies and Research Topics,” Reviews in Economic Geology Vol. 6B, Society of Economic Geologists, Inc., 373-432.
Postgate, J.R., 1965, “Recent advances in the study of sulfate-reducing bacteria,” Bacteriological Reviews, Vol. 29, No. 4, pp. 425-441.
Postgate, J.R., 1974, “Genus Desulfovibrio. In: Bergey's manual of determinative bacteriology (R. E. Buchanan, N. E. Gibbons, ed.), 8^thed.,” Baltimore: Williams and Wilkins, pp. 418-421.
Reinertsen, S.A., Elliott, L.F., Cochran, V.L. and Campbell, G.S., 1984, “Role of available carbon and nitrogen in determining the rate of wheat straw decomposition,” Soil Biol. Biochem., Vol. 16, pp. 459-464.
Sheoran, A.S., Sheoran, V. and Choudhary, R.P., 2010, “Bioremediation of acid-rock drainage by sulphate-reducing prokaryotes: A review,” Minerals Engin., Vol. 23, pp. 1073-1100.
Song, Y., Fitch, M., Burken, J., Nass, L., Chilukiri, S., Gale, N. and Ross, C., 2001, “Lead and zinc removal by laboratory- scale constructed wetlands,” Water Environ. Res., Vol. 73, pp. 37-44.
Utgikar, V.P., Chaudhary, N., Koeniger, A., Tabak, H.H., Haines, J.R. and Govind, R., 2004, “Toxicity of metals and metal mixtures: Analysis of concentration and time dependence for zinc and copper,” Water Res., Vol. 38, pp. 3651-3658.
Younger, P.L, Jayaweera, A., Elliot, A., Wood, R., Amos, P. and Daugherty, A.J., 2003, “Passive treatment of acidic mine waters in subsurface flow systems: exploring RAPS and permeable reactive barriers,” Land Contam Reclam, Vol. 11, pp. 127-135.
Zagury, G.J., Kulnieks, V. and Neculita, C.M., 2006, “Charaterization and reactivity assessment of organic substrates for sulfate reducing bacteria in acid mine drainage treatment,” Chemosphere, Vol. 64, pp. 944-954.
Ziemkiewicz, P.F., Skousen, J.G. and Simmons, J., 2003, “Long-term performance of passive acid mine drainage treatment systems,” Mine Water Environ., Vol. 22, pp. 118- 129.

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 53
No :5
Pages :387-397
DOI :https://doi.org/10.12972/ksmer.2016.53.5.387

[1] APHA, 1998, “Standard Methods for the Examination of Water and Wastewater, In: Clesceri, L.S., Greenberg, A.E., Eaton, A.D.(Eds.),” Washington, DC.

[2] Be´chard, G., Yamazaki, H., Gould, W.D. and Be´dard, P., 1994, “Use of cellulosic substrates for the microbial treatment of acid mine drainage,” J. Environ. Qual. Vol. 23, pp. 111-116.

[3] Chen, B.Y., Utgikar, V.P., Harmon, S.M., Tabak, H.H., Bishop, D.F. and Govind, R. 2000, “Studies of biosorption of zinc(II) and copper(II) on Desulfovibrio desulfuricans,” Int. Biodeterior. Biodegrad., Vol. 46, pp. 11-18.

[4] Clark D.S., 1967, “Comparison of pour and surface plate methods for determination of bacterial count,” Canadian Journal of Microbiology, Vol. 13, No. 11, pp. 1409-1412.

[5] David, J.R., Vijayakumar S., Souhail R. Al-Abeda, Derrick, A., 2007, “Statistical validation of sulfate quantification methods used for analysis of acid mine drainage,” Talanta, Vol. 71, No. 1, pp. 303-311.

[6] Gazea, B., Adam, K. and Kontopoulos., A. 1996, “A review of passive systems for the treatment of acid mine drainage,” Miner. Eng., Vol. 9, pp. 23-42.

[7] Gray, N.F. and O’'Neill, C., 1997, “Acid mine drainage toxicity testing,” Environ. Geochem. Health, Vol. 19, pp. 165-171.

[8] Hulshof, A.H.M., Blowes, D.W., Ptacek, C.J. and Gould, W.D., 2003, “Microbial and nutrient investigations into the use of in situ layers for treatment of tailings effluent,” Environ. Sci. Technol., Vol. 37, pp. 5027-5033.

[9] Jong, T. and Parry, D.L., 2003, “Removal of sulfate and heavy metals by sulfate reducing bacteria in short-term bench scale upflow anaerobic packed bed reactor runs,” Water Research, Vol. 37, pp. 3379-3389.

[10] Jong, T. and Parry, D.L., 2004, “Heavy metal speciation in solid-phase materials from abacterial sulfate reducing bioreactor using sequential extraction procedure combined with acid volatile sulfide analysis,” J. Environ. Monit., Vol. 6, pp. 278-285.

[11] Lens, P., Vallero, M., Esposito, G. and Zandvoort, M., 2002, “Perspectives of sulfate reducing bioreactors in environmental biotechnology,” Re/Views in Environmental Science and Bio/Technology, Vol. 1, pp. 311-325.

[12] Logan, M.V., Reardon, K.F., Figueroa, L.A., McLain, J.E.T. and Ahmann, D.M., 2005, “Microbial community activities during establishment, performance, and decline of bench- scale passive treatment systems for mine drainage,” Water Research, Vol. 39, pp. 4537-4551.

[13] Manabu, F. and Susumu, T., 1989, “Reduction of tetrazolium salts by sulfate-reducing bacteria,” FEMS Microbiology Ecology, Vol. 5, No. 1, pp. 13-19.

[14] Perry, R.H. and Green, D., 1984, “Perry’s Chemical Engineer’s Handbook, sixth ed.,” McGraw-Hill Book Company, Singapore.

[15] PIRAMID Consortium, 2003, “Engineering guidelines for the passive remediation of acidic and metalliferous mine drainage and similar wastewaters,” European Commission 5th Framework RTD Project EVK1-CT-1999-000021, University of Newcastle Upon Tyne, Newcastle Upon Tyne, UK.

[16] Plumlee, G.S., Smith, K.S., Montour, M.R., Ficklin, W.H. and Mosier, E.L., 1999, “Geologic Controls on the Composition of Natural Waters and Mine Waters Draining Diverse Mineral-Deposit Types. In: L.H. Filipek and G.S. Plumlee (Eds.), The Environmental Geochemistry of Mineral Deposits, Part B: Case Studies and Research Topics,” Reviews in Economic Geology Vol. 6B, Society of Economic Geologists, Inc., 373-432.

[17] Postgate, J.R., 1965, “Recent advances in the study of sulfate-reducing bacteria,” Bacteriological Reviews, Vol. 29, No. 4, pp. 425-441.

[18] Postgate, J.R., 1974, “Genus Desulfovibrio. In: Bergey's manual of determinative bacteriology (R. E. Buchanan, N. E. Gibbons, ed.), 8^thed.,” Baltimore: Williams and Wilkins, pp. 418-421.

[19] Reinertsen, S.A., Elliott, L.F., Cochran, V.L. and Campbell, G.S., 1984, “Role of available carbon and nitrogen in determining the rate of wheat straw decomposition,” Soil Biol. Biochem., Vol. 16, pp. 459-464.

[20] Sheoran, A.S., Sheoran, V. and Choudhary, R.P., 2010, “Bioremediation of acid-rock drainage by sulphate-reducing prokaryotes: A review,” Minerals Engin., Vol. 23, pp. 1073-1100.

[21] Song, Y., Fitch, M., Burken, J., Nass, L., Chilukiri, S., Gale, N. and Ross, C., 2001, “Lead and zinc removal by laboratory- scale constructed wetlands,” Water Environ. Res., Vol. 73, pp. 37-44.

[22] Utgikar, V.P., Chaudhary, N., Koeniger, A., Tabak, H.H., Haines, J.R. and Govind, R., 2004, “Toxicity of metals and metal mixtures: Analysis of concentration and time dependence for zinc and copper,” Water Res., Vol. 38, pp. 3651-3658.

[23] Younger, P.L, Jayaweera, A., Elliot, A., Wood, R., Amos, P. and Daugherty, A.J., 2003, “Passive treatment of acidic mine waters in subsurface flow systems: exploring RAPS and permeable reactive barriers,” Land Contam Reclam, Vol. 11, pp. 127-135.

[24] Zagury, G.J., Kulnieks, V. and Neculita, C.M., 2006, “Charaterization and reactivity assessment of organic substrates for sulfate reducing bacteria in acid mine drainage treatment,” Chemosphere, Vol. 64, pp. 944-954.

[25] Ziemkiewicz, P.F., Skousen, J.G. and Simmons, J., 2003, “Long-term performance of passive acid mine drainage treatment systems,” Mine Water Environ., Vol. 22, pp. 118- 129.

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