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2020 Vol.57, Issue 3 Preview Page

Technical Report

Field Applicability Field Applicability of Heavy Metal Extraction Technique from Contaminated Soils using Sulfur or Iron Oxidizing Bacteria

황 및 철산화균을 이용한 오염토양 중금속 용출 기술의 현장적용성을 위한 연구

Hyeop-Jo Han1
,
Myoung-Soo Ko2
,
Hyun-Sung Park3
,
Jong-Un Lee1*
한 협조1
,
고 명수2
,
박 현성3
,
이 종운1*
1Department of Energy and Resources Engineering, Chonnam National University
2Department of Energy and Resources Engineering, Kangwon National University
3Honam Regional Office, Mine Reclamation Corporation
1전남대학교 에너지자원공학과
2강원대학교 에너지자원‧산업공학부 에너지자원공학전공
3한국광해관리공단 호남지사
* Corresponding Author
30 June 2020. pp. 275-285
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Abstract

Most studies on the bioleaching of heavy metals from contaminated soils using sulfur or iron-oxidizing bacteria have been conducted in laboratories under experimental conditions favorable for microbial activity. In this study, the applicability of bioleaching to field was investigated by examining various technical problems. Under the condition of a high solid-liquid ratio up to 1:3, the bioleaching showed a satisfactory efficiency. It resulted in high amounts of extracted heavy metals from fine soil particles under 200 mesh, and agitation of soil slurry led to an enhanced efficiency. For the preparation of a large volume of bacterial enrichment up to 1,000 L, a sequential increase of culture medium volume showed positive results, and subculture of bacteria in the final medium volume led to increase in bacterial activity. An input amount of Fe2+ less than 9 g/L also resulted in high bioleaching efficiency, thus a preliminary determination of appropriate Fe2+ amount may be required prior to field application.

Keywords
Bioleaching
Soil
Heavy metals
Field applicability
Sulfur or iron-oxidizing bacteria

황 및 철산화균을 이용하여 오염 토양으로부터 중금속을 용출한 그간의 연구는 대부분 실험실에서 미생물 활성에 유리한 조건을 조성한 상태에서 수행되었다. 이 연구는 미생물학적 용출법을 현장 토양에 적용할 때 직면하는 기술적 문제들을 조사함으로써 현장적용성을 높이기 위한 목적으로 수행되었다. 반응 슬러리의 고액비를 1:3 정도로 대폭 증가시켜도 효과적인 중금속 용출이 관찰되었다. 미생물학적 용출은 -200 mesh의 미립질 토양으로부터도 향상된 중금속 용출을 보였으며 특히 슬러리를 교반하였을 경우 한층 효과적이었다. 1,000 L에 이르는 대용량 배양조에서 미생물을 증균할 경우 순차적으로 용량을 증가시키며 배양하는 것이 효율적이며 최종 용량에서는 계대배양을 반복할수록 미생물의 활성도가 증가하였다. 철산화균의 에너지원으로 필요한 Fe2+ 투입량은 표준 용량인 9 g/L보다 적어도 높은 효율을 보이므로 현장 적용 전 사전 연구를 통해 적정 투입량을 결정하는 것이 필요하다.

키워드
미생물학적 용출
토양
중금속
현장적용성
황 및 철산화균
References
1
Borja, D., Lee, E., Silva, R.A., Kim, H., Park, J.H., and Kim, H., 2015. Column bioleaching of arsenic from mine tailings using a mixed acidophilic culture: A technical feasibility assessment. J. Kor. Inst. Resources Recycling, 24(6), p.69-77.
10.7844/kirr.2015.24.6.69
2
Couillard, D. and Zhu, S., 1991. bacterial leaching of heavy metals from sewage sludge for agricultural application. Water Air Soil Pollut., 63(1-2), p.67-80.
10.1007/BF00475622
3
Feng, G., Xie, T., Wang, X., Bai, J., Tang, L., Zhao, H., Wei, W., Wang, M., and Zhao, Y., 2018. Metagenomic analysis of microbial community and function involved in cd-contaminated soil. BMC Microbiol., 18(1), p.11.
10.1186/s12866-018-1152-529439665PMC5812035
4
Geng, H., Wang, F., Yan, C., Tian, Z., Chen, H., Zhou, B., Yuan, R., and Yao, J., 2020. Leaching behavior of metals from iron tailings under varying pH and low molecular- weight organic acids. J. Hazard. Mater., 383, p.121136.
10.1016/j.jhazmat.2019.12113631525690
5
Gupta, A., Dutta, A., Sarkar, J., Paul, D., Panigrahi, M.K., and Sar, P., 2017. Metagenomic exploration of microbial community in mine tailings of Malanjkhand copper project, India. Genom. Data, 12, p.11-13.
10.1016/j.gdata.2017.02.00428239550PMC5315440
6
Han, H.-J. and Lee, J.-U., 2015. Experimental study on bioleaching of paddy soil in the vicinity of refinery site contaminated with copper, lead, and arsenic using sulfur- oxidizing bacteria. Geosys. Eng., 18(2), p.79-84.
10.1080/12269328.2014.1002634
7
Han, H.-J., Lee, J.-U., and Chon, H.-T., 2011. Comparison of bioleaching of heavy metals and arsenic from contaminated soil in the vicinity of a refinery using sulfur-oxidizing and iron-oxidizing bacteria. J. Korean Soc. Geosys. Eng., 48(6), p.713-722.
8
Han, H.-J., Lee, J.-U., Ko, M.-S., Choi, N.-C., Kwon, Y.-H., Kim, B.-K., and Chon, H.-T., 2009. Bioleaching of heavy metals from shooting range soil using a sulfur-oxidizing bacteria Acidithiobacillus thiooxidans. Econ. Environ. Geol., 42(5), p.457-469.
9
Kedziorek, M.A., Dupuy, A., Bourg, A.C., and Compère, F., 1998. Leaching of Cd and Pb from a polluted soil during the percolation of EDTA: laboratory column experiments modeled with a non-equilibrium solubilization step. Environ. Sci. Technol., 32(11), p.1609-1614.
10.1021/es970708m
10
Kim, Y.-S., Chon, H.-T., and Lee, J.-U., 2011. Bioleaching of heavy metals and arsenic in contaminated soil by microbiological sulfur oxidation. J. Korean Soc. Geosys. Eng., 48(3), p.294-308.
11
Ko, M.-S., Park, H.-S., and Lee, J.-U., 2009. Bioleaching of heavy metals from tailings in abandoned Au-Ag mines using sulfur-oxidizing bacterium Acidithiobacillus thiooxidans. J. Korean Soc. Geosys. Eng., 46(2), p.239-251.
12
Ko, M.-S., Park, H.-S., Kim, K.-W., and Lee, J.-U., 2013. The role of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in arsenic bioleaching from soil. Environ. Geochem. Health, 35(6), p.727-733.
10.1007/s10653-013-9530-223709230
13
Lee, J.-U., Kim, S.-M., Kim, K.-W., and Kim, I.S, 2005. Microbial removal of uranium in uranium-bearing black shale. Chemosphere, 59(1), p.147-154.
10.1016/j.chemosphere.2004.10.00615698655
14
Lee, K.Y., Kim, K.R., Lee, B.T., Kim, J.Y., Kim, K.W., and Kim, S.O., 2009. Evaluation on the feasibility of microbially enhanced electrokinetic removal of multiple heavy metals from tailing soil. Sep. Sci. Technol., 44(10), p.2322-2340.
10.1080/01496390902983653
15
Mardanov, A.V., Beletsky, A.V., Ivasenko, D.A., Karnachuk, O.V., and Ravin, N.V., 2017. Metagenome sequence of a microbial community from the gold mine tailings in the Kuzbass area, Russia. Genome Announc., 5(49), e01355-17.
10.1128/genomeA.01355-1729217798PMC5721143
16
Nguyen, V.K. and Lee, J.-U., 2015. A comparison of microbial leaching and chemical leaching of arsenic and heavy metals from mine tailings. Biotechnol. Bioprocess. Eng., 20(1), p.91-99.
10.1007/s12257-014-0223-1
17
Park, H.-S., Lee, J.-U., and Ahn, J.-W., 2007. The effects of Acidithiobacillus ferrooxidans on the leaching of cobalt and strontium adsorbed onto soil particles. Environ. Geochem. Health, 29(4), p.303-312.
10.1007/s10653-007-9095-z17508258
18
Seh-Bardan, B.J., Othman, R., Wahid, S.A., Husin, A., and Sadegh-Zadeh, F., 2012. Column bioleaching of arsenic and heavy metals from gold mine tailings by Aspergillus fumigatus. CLEAN Soil Air Water, 40(6), p.607-614.
10.1002/clen.201000604
19
Tunsu, C., Menard, Y., Eriksen, D.Ø., Ekberg, C., and Petranikova, M., 2019. Recovery of critical materials from mine tailings: A comparative study of the solvent extraction of rare earths using acidic, solvating and mixed extractant systems. J. Clean. Prod., 218, p.425-437.
10.1016/j.jclepro.2019.01.312
20
Tyagi, R.D. and Couillard, D., 1989, Bacterial leaching of metals from sludge, In: Cheremisinoff, P.E. (Ed), Encyclopedia of Environmental Control Technology, Vol. 3, Gulf Publishing Co., Houston, p.557-591.
21
Wasay, S.A., Barrington, S.F., and Tokunaga, S., 1998. Remediation of soils polluted by heavy metals using salts of organic acids and chelating agents. Environ. Technol., 19(4), p.369-379.
10.1080/09593331908616692
22
Zhang, X., Niu, J., Liang, Y., Liu, X., and Yin, H., 2016. Metagenome-scale analysis yields insights into the structure and function of microbial communities in a copper bioleaching heap. BMC Genet., 17(1), p.21.
10.1186/s12863-016-0330-426781463PMC4717592
Information
  • Publisher :The Korean Society of Mineral and Energy Resources Engineers
  • Publisher(Ko) :한국자원공학회
  • Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
  • Journal Title(Ko) :한국자원공학회지
  • Volume : 57
  • No :3
  • Pages :275-285
  • Received Date : 2020-06-05
  • Revised Date : 2020-06-23
  • Accepted Date : 2020-06-25
  • DOI :https://doi.org/10.32390/ksmer.2020.57.3.275
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