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2011 Vol.48, Issue 1 Preview Page
The Corrosion and the Enhance of Bioleaching for Galena by Moderate Thermophilic Indigenous Bacteria

고온성토착박테리아에 의한 방연석의 충식작용과 용출 향상

박천영, 정경훈, 김봉주, 위환, 이윤국

Cheon-Young Park, Kyung-Hoon Cheong, Bong-Ju Kim, Hwan Wi and Youn-Goog Lee

28 February 2011. pp. 11-24
PDF Citation
Abstract
This study was to investigate the viability of indigenous acidophilic bacteria at 42℃ and 52℃, and to enhance the leaching of valuable elements from galena during the oxidation of the indigenous acidophilic bacteria with no addition of sulfuric acid. In the zeta potential results for the galena, the isoelectric point obtained was at a pH value of 2.43. Even through the isoelectric point of the pH environment was higher, the indigenous bacteria were attached to the surface of the galena. In the bioleaching of galena at 42℃ and 52℃, the rod-shaped bacteria ranged from 0.4×1.3 ㎛ to 0.5×1.6 ㎛ and were attached to the galena surface. Corrosion pits were distinctly observed on the surface of the secondary mineral by the direct contact attachment of indigenous bacteria. This is evidence that the direct contact oxidation mechanism of the bacteria were directly attached to oxidize the surface of the sulfide mineral. With the increase of 10℃ from 42℃ to 52℃ in the bioleaching experiment, the content of and ions increased up to 3.7 and 5.2 times, respectively, whereas the content of was decreased by up to 2.6 times.
Keywords
Moderate thermophilic indigenous bacteria
Galena
Extracellular polymeric substance
Bioleaching
Corrosion pit
고온성토착박테리아를 이용하여 방연석으로부터 용출되는 유용금속이온의 향상을 위하여 42℃와 52℃에서 용출실험을 수행하였다. 용출실험은 황산을 첨가하지 않고 수행하였다. 방연석에 대한 제타전위를 측정한 결과 방연석의 등전위는 2.43으로 나타났다. 방연석의 등전위 보다 높은 pH 환경임에도 불구하고 토착박테리아들이 방연석 표면에 부착하였다. 42℃와 52℃로 미생물용출실험을 진행하는 동안 0.4×1.3 ㎛에서부터 0.5×1.6 ㎛ 크기의 토착박테리아들이 방연석 표면에 부착된 것이 관찰되었다. 2차 광물 표면에 토착박테리아에 의하여 형성된 충식자국들이 뚜렷하게 관찰되었다. 이것은 박테리아가 황화광물 표면에 직접 접촉하여 산화작용을 일으키는 직접접촉산화작용의 증거로 생각된다. 미생물 용출온도를 42℃에서 52℃로 증가시켰을 때, 는 3.7배 이상으로 용출되었고 그리고 는 5.2배 이상으로 용출되었다. 하지만, Pb는 2.6배 감소하였다.
키워드
고온성토착박테리아
방연석
세포외중합체물질
미생물용출
충식
References
  1. 고명수, 박현성, 이종운, 2009, “황산화균 Acidithiobacillus thiooxidans를 이용한 폐금은광산 광미에서의 중금속 용출,” 한국지구시스템공학회지, 제46권 2호, pp. 228-238.
  2. 박천영, 김순오, 김봉주, 2010, “42℃에서 토착호산성박테리아의 황철석 표면에 대한 선택적 부착과 용출 특성,” 자원환경지질, 제43권 2호, pp. 109-121.
  3. 박천영, 윤정한, 박영석, 1989, “광양금은광상의 성인에 관한 연구,” 한국자원공학회지, 제26권 4호, pp. 249-263.
  4. 박천영, 정경훈, 김계민, 홍영의, 조강희, 2009, “화순 광산 배수에 서식하는 토착 호산성 박테리아를 이용한 황철석의 용출 특성,” 한국지구시스템공학회지, 제46권 2호, pp. 521-535.
  5. 박천영, 조강희, 2010, “토착호산성박테리아의 황철석 표면 부착과 용출 특성,” 한국지구시스템공학회지, 제47권 1호, pp. 51-60.
  6. Ahonen, L. and Tuovinen, O.H., 1989, “Microbiology oxidation of ferrous iron at low temperatures,” Applied and Environmental Microbiology, Vol. 55, pp. 312-316.
  7. Ahonen, L. and Tuovinen, O.H., 1990, “Kinetics of sulfur oxidation at suboptimal temperature,” Applied and Environmental Microbiology, Vol. 56, pp. 560-562.
  8. Ahonen, L. and Tuovinen, O.H., 1991, “Temperature effects on bacterial leaching of sulfide minerals in shake flask experiments,” Applied and Environmental Microbiology, Vol. 57, pp. 138-145.
  9. Attia, Y.A. and El-Zeky, M., 1990, “Effects of galvanic interactions of sulfides on extraction of percious metals from refractory complex sulfides by bioleaching,” International Journal of Mineral Processing, Vol. 30, pp. 99-111.
  10. Attia, Y.A. and El-Zeky, M.A., 1990, “Bioleaching of nonferrous sulfides with adapted thiophillic bacteria,” The Chemical Engineering Journal, Vol. 44, pp. B31-B40.
  11. Bang, S.S., Deshpande, S.S. and Han, K.N., 1995, “The oxidation of galena using Thiobacillus ferrooxidans,” Hydrometallurgy, Vol. 37, pp. 181-192.
  12. Bhatti, T.M., Bigham, J.M., Carlson, L. and Tuovinen, O.H., 1993, “Mineral products of pyrrhotite oxidation by Thiobacillus ferrooxidans,” Applied and Environmental Microbiology, Vol. 59, pp. 1984-1990.
  13. Boon, M., 2001, “The mechanism of ‘direct’ and 'indirect' bacterial oxidation of sulfide minerals,” Hydrometallurgy, Vol. 62, pp. 67-70.
  14. Brierley, C.L., 1978a, “Bacterial leaching,” Critical Reviews in Microbiology, Vol. 6, pp. 207-262.
  15. Brierley, C.L., 1982, “Microbiological mining,” Scientific American, Vol. 247, pp. 42-51.
  16. Brierley, J.A. and Brierley, C.L., 2001, “Present and future commercial applications of biohydrometallurgy,” Hydrometallurgy, Vol. 59, pp. 233-239.
  17. Brierley, J.A., 1978b, “Thermophilic iron-oxidising bacteria found in copper leaching dumps,” Applied and Environmental Microbiology, Vol. 36, pp. 523-525.
  18. Brierley, J.A., 2003, “Response of microbial systems to thermal stress in heap-biooxidation pretreatment of refractory gold ores,” Hydrometallurgy, Vol. 71, pp. 13-19.
  19. Brock, T.D., 1986, “Introduction: an overview of the thermophiles,” In Brock, T.D.(eds), Thermophiles, John Wiley & Sons, pp. 1-16.
  20. Bruynesteyn, A. and Duncan, D.W., 1971, “Microbiological leaching of sulfide minerals,” Canadian Metallurgical Quarterly, Vol. 10, pp. 57-63.
  21. da Silva, G., 2004, “Kinetics and mechanism of the bacterial and ferric sulphate oxidation of galena,” Hydrometallurgy, Vol. 75, pp. 99-110.
  22. da Silva, G., Lastra, M.R. and Budden, J.R., 2003, “Electrochemical passivation of sphalerite during bacterial oxidation in the presence of galena,” Mineral Engineering, Vol. 16, pp. 199-203.
  23. Dave, S.R., Natarajan, K.A. and Bhat, J.V., 1979, “Biooxidation studies with T. ferrooxidans in the presence of copper and zinc,” Trans. Inst. Min. Metall., Vol. 88, pp. C234-C237.
  24. Escobar, B., Huerta, G. and Rubio, J., 1997, “Short communication: influence of LPS on the attachment of Thiobacillus ferrooxidans to minerals,” World Journal of Microbiology & Biotechnology, Vol. 13, pp. 593-594.
  25. Fletcher, A.W., 1970, “Metal winning from low-grade ore by bacterial leaching,” Trans. Inst. Min. Metall., Vol. 79, pp. C247-C252.
  26. Garcia, O., Jr. Bigham, J.M. and Tuovinen, O.H., 1995a, “Oxidation of galena by Thiobacillus ferrooxidans and Thiobacillus thiooxidans,” Canadian Journal of Microbiology, Vol. 41, pp. 508-514.
  27. Garcia, O. Jr., Bigham, J.M. and Tuovinen, O.H., 1995b, “Sphalerite oxidation by Thiobacillus ferrooxidans and Thiobacillus thiooxidans,” Canadian Journal of Microbiology, Vol. 41, pp. 578-584.
  28. Gehrke, T., Telegdi, J., Thierry, D. and Sand, W., 1998, “Importance of extracellular polymeric substances from Thiobacillus ferrooxidans for bioleaching,” Applied and Environmental Microbiology, Vol. 64, pp. 2743-2747.
  29. Gomez, C., Blazquez, M.L. and Ballester, A., 1999, “Bioleaching of Spanish complex sulphide ore bulk concentrate,” Minerals Engineering, Vol. 12, pp. 93-106.
  30. Gormely, L.S., and Duncan, D.W., 1974, “Estimation of Thiobacillus ferrooxidans Concentrations,” Canadian Journal of Microbiology, Vol. 20, pp. 1453-1455.
  31. Grishin, S.I., Bigham, J.M. and Tuovinen, O.H., 1988, “Characterization of jarosite formed upon bacterial oxidation of ferrous sulfate in a packed-bed reactor,” Applied and Environmental Microbiology, Vol. 54, pp. 3101-3106.
  32. Harden, V.P. and Harris, J.O., 1953, “The isoelectric point of bacterial cells,” J. Bacteral., Vol. 65, pp. 198-202.
  33. Kinzler, K., Gehrke, T., Telegdi, J. and Sand, W., 2003, “Bioleaching - a result of interfacial processes caused by extracellular polymeric substances (EPS),” Hydrometallurgy, Vol. 71, pp. 83-88.
  34. Konhauser, K., 2007, Introduction to geomicrobiology, Blackwell Publishing, p. 425.
  35. Lottermoser, B., 2007, Mine wastes, Springer, p. 304.
  36. Malouf, E.E. and Prater, J.D., 1961, “Role of bacteria in the alteration of sulfide minerals,” Journal of Metals, Vol. 13, pp. 353-356.
  37. Marsden, J. and House, I., 1992, The chemistry of gold extraction, Ellis Horwood, p. 597.
  38. Marshal, K.C., 1976, Interface in microbial ecology, Havard University Press, London, pp. 27-52.
  39. Mousavi, S.M., Taghmaei, S., Vossoughi, M., Jafari, A. and Hoseini, S.A., 2005, “Comparation of bioleaching ability of two native mesophilic and thermophilic bacteria on copper recovery from chalcopyrite concentrate in an airlift bioreactor,” Hydrometallurgy, Vol. 80, pp. 139-144.
  40. Poliani, C. and Donati, E., 1999, “The role of exopolymers in the bioleaching of a non-ferrous metal sulphide,” Journal of Industrial Microbiology & Biotechnology, Vol. 22, pp. 88-92.
  41. Rodriguez-Leiva, M. and Tributsch, H., 1988, “Morphology of bacterial leaching patterns by Thiobacillus ferrooxidans on synthetic pyrite,” Archives of Microbiology, Vol. 149, pp. 401-405.
  42. Rojas-Chapana, J.A., Giersig, M. and Tributsch, H., 1995, “Sulfur colloids as temporary energy reservoirs for Thiobacillus ferrooxidans during pyrite oxidation,” Archives of Microbiology, Vol. 163, pp. 352-356.
  43. Sand, W., Gehrke, T., Hallmann, R. and, Schippers, A., 1995, “Sulfur chemistry, biofilm, and the (in)direct attack mechanism-a critical eveluation of bacterial leaching,” Applied Microbiology and Biotechnology, Vol. 43, pp. 961-966.
  44. Sand, W., Gehrke, T., Jozsa, P.G. and Schippers, A., 2001, “(Bio)chemistry of bacterial leaching - direct vs indirect bioleaching,” Hydrometallurgy, Vol. 59, pp. 159-175.
  45. Santhiya, D., Subramanuan, S. and Natarajan, K.A., 2001, “Bio-modulation of galena and sphalerite surface using Thiobacillus thiooxidans,” International Journal of Mineral Processing, Vol. 62, pp. 121-141.
  46. Schippers, A., and Sand, W., 1999, “Bacterial leaching of metal sulfides proceeds by two in direct mechanisms via thiosulfate of via polysulfides and sulfur,” Applied and Environmental Microbiology, Vol. 65, pp. 319-321.
  47. Schippers, A., 2007, “Microorganisms involved in bioleaching and nucleic acid-based molecular methods for their identification and quantification,” In Donati, E. R. and Sand, W. (eds), Springer, pp. 3-33.
  48. Silverman, M. P., 1967, “Mechanism of bacteria pyrite oxidation,” Journal of Bacteriology, Vol. 94, pp. 1046-1051.
  49. Silverman, M.P. and Ehrlich, H.L., 1964, “Microbial formation and degradation of minerals,” Advan. Appl. Microbiol., Vol. 6, pp. 153-206.
  50. Torma, A.E. and Habashi, F., 1972, “Oxidation of copper (II) selenide by Thiobacillus ferrooxidans,” Canadian Journal of Microbiology, Vol. 18, pp. 1780-1781.
  51. Torma, A.E., 1977, “The role of Thiobacillus ferrooxidans in hydrometallurgical process,” Adv. Biochem. Eng., Vol. 6, pp. 1-37.
  52. Tributsch, H., 2001, “Direct versus indirect bioleaching,” Hydrometallurgy, Vol. 59, pp. 177-185.
  53. Tuovinen, O.H., Bhatti, T.M., Bigham, J.M., Hallberg, K.B., Garcia, Jr., O. and Lindstrom, E.B., 1994, “Oxidative dissolution of arsenopyrite by mesophilic and moderately thermophilic acidophiles,” Applied and Environmental Microbiology, Vol. 60, pp. 3268-3274.
  54. Wolfaardt, G.M., lawrence, J.R. and Korber, D.R., 1999, “Functions of EPS,” In Jost, W. (eds), Microbial extracellular polymeric substance: characterization, structure, and functions, Springer, pp. 171-200.
  55. Yelloji Rao, M.K., Natarajan, K.A. and Somasundaran, P., 1992, “Effect of biotreatment with Thiobacillus ferrooxidans on the floatability of sphalerite and galena,” Mineral & Metallurgical Processing, Vol. 9, pp. 95-100.
  56. Yu, J. Y., McGenity, T. J. and Coleman, M. L., 2001, “Solution chemistry during the lag phase and exponential phase of pyrite oxidation by Thiobacillus ferrooxidans,” Chemical Geology, Vol. 175, pp. 307-317.
Information
  • Publisher :The Korean Society of Mineral and Energy Resources Engineers
  • Publisher(Ko) :한국자원공학회
  • Journal Title :Journal of the Korean Society for Geosystem Engineering
  • Journal Title(Ko) :한국지구시스템공학회지
  • Volume : 48
  • No :1
  • Pages :11-24
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