Analysis of Microorganisms in Petroleum Reservoir by Molecular Biological Approach

doi:10.12972/ksmer.2014.51.3.404

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2014 Vol.51, Issue 3 Preview Page Next Page

Research Paper

Analysis of Microorganisms in Petroleum Reservoir by Molecular Biological Approach 분자생물학적 방법에 의한 석유 저류층 미생물 군집 분석: 임경철·김현태·백경화·김진수·손한암
Kyung Chul Im, Hyun Tae Kim, Kyung Hwa Baek, Jin Soo Kim, Han Am Son

30 June 2014. pp. 404-414

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Abstract

The purpose of this study is to analyze the dominant microorganisms in oil reservoirs to apply for MEOR. Oilsand from Canada and mid-heavy from Myanmar were sampled for the research, and the microorganisms cluster analysis was carried out by molecular biological approach. The research started with DNA extraction and amplification from the samples, and analyzed the DNA for dominant species. Throughout the research, it was able to confirm the appropriate dominant microorganisms for MEOR in Canada and Myanmar. For oilsand in Canada, Streptococcus, microorganism which degrades hydrocarbon to separate oil and sand by using produced organic acid and bio gas, was found. In reservoir of Myanmar, and Clostridium was found that it is able to improve immobility of oil by decreasing oil viscosity from CO₂, H₂, CH₄ produced. The processes from the research can be utilized to develop MEOR techniques in the future.

Keywords

MEOR

Dominant microorganisms

Microorganisms cluster analysis

Molecular biological approach

DNA extraction and amplification

이 연구는 미생물을 이용한 석유회수증진기술에 적용 가능한 우점종 미생물을 찾아내기 위해 수행하였다. 미생물 시료는 캐나다 오일샌드와 미얀마 육상유전 것을 사용하였으며, 미생물 군집 분석은 최신 분자생물학적 방법인 파이로시퀀싱을 사용하였다. 실험은 먼저 시료로부터 저류층 미생물의 DNA를 추출한 후, 증식과정을 거쳐 우점종을 파악하였다. 실험결과 캐나다 오일샌드 지역과 미얀마 육상유전 지역의 MEOR 적용 가능한 우점종 미생물을 확인할 수 있었다. 캐나다 오일샌드 지역은 유기산과 바이오가스 등이 산출되어 탄산을 용해시켜 오일과 암석을 쉽게 분리시킬 수 있는 Streptococcus 미생물이 발견되었고, 미얀마 유전은 이산화탄소, 수소, 메탄 등이 생성되어 오일의 점도를 낮추어 주는 Clostridium 미생물이 발견되었다. 이 연구에서 정립된 미생물 군집 분석 공정은 향후, MEOR 기술개발에 활용될 수 있다.

키워드

미생물을 이용한 석유회수증진기술

우점종 미생물

미생물 군집 분석

분자생물학적 방법

DNA 추출 및 증식

References

Al-Sulaimani, H., Joshi, S., Al-Wahaibi, Y., Al-Bahry, S., Elshafie, A. and Al-Bemani, A., 2011, “Microbial Biotechnology for Enhancing Oil Recovery: Current Developments and Future Prospects,” Biotechnology, Bioinformatics and Bioengineering, Vol. 1, No. 2, pp. 147-158.
Aladasani, A. and Bai, B., 2010, “Recent Developments and Updated Screening Criteria of Enhanced Oil Recovery Techniques,” SPE Paper 103726, Proc. of CPS/SPE International Oil & Gas Conference and Exhibition, Beijing, China, June 8-10.
Bock, M., Kampfer, P., Bosecker, K. and Dott, W., 1994, “Isolation and Characterization of Heterotrophic, Aerobic Bacteria from Oil Storage Caverns in Northern Germany,” Applied Microbiology and Biotechnology, Vol. 42, pp. 463-468.
Cole, J.R., Chai, B., Farris, R.J., Wang, Q., Kulam, S.A., McGarrell, D.M., Garrity, G.M. and Tiedje, J.M., 2005, “The Ribosomal Database Project (RDP-II): Sequences and Tools for Highthroughput rRNA Analysis,” Nucleic Acids Research, Vol. 33, pp. 294-296.
El Ela, M.A., El-Tayeb, S., Sayyouh, M.H., Abdel Dayem, M. and Desouky, S., 2002, “Effects of Stimulating Indigenous Bacteria in Oil Reservoirs on Relative Permeability Curves,” SPE Paper 75240, Proc. of SPE/DOE Improved Oil Recovery Symposium, Tulsa, Oklahoma, April 13-17.
Harner, N.K., Richardson, T.L., Thompson, K.A., Best, R.J., Best, A.S. and Trevors, J.T., 2011, “Microbial Processes in the Athabasca Oil Sands and Their Potential Applications in Microbial Enhanced Oil Recovery,” J. of Industrial Microbiology & Biotechnology, Vol. 38, pp. 1761-1775.
Haiying, C. and Weidong, W., 2007, “16S rRNA: Genes Comparative Analysis of Microbial Communities in Oil Reservoir With Nutrients Injection by Terminal Restriction Fragment Length Polymorphism Method,” SPE Paper 106398, presented at SPE Internatinoal Symposium on Oilfield Chemistry, Houston, Texas, Februry 28-March 2.
Hitzman, D.O., Dennis, M. and Hitzman, D.C., 2004, “Recent Successes: MEOR Using Synergistic H2S Prevention and Increased Oil Recovery Systems,” SPE Paper 89453, Proc. of 2004 SPE/DOE Fourteenth Symposium on Improved Oil Recovery, Tulsa, Oklahoma, April 17-21.
Kim, E.S., Hong, S.W. and Chung, K.S., 2011, “Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm using DGGE and Pyrosequencing,” Korean J. of Microbiology and Biotechnology, Vol. 39, No. 4, pp. 374-381.
Kim, K. and Kim, D.A., 2013, “The Comparison Between Serologic Tests and Magicplex HepaTrio Real-time PCR in the Diagnosis of Viral Hepatitis,” Laboratory Medicine Online, Vol. 3, No. 1, pp. 23-28.
Kotlar, H.K., Brakstad, O.G. and Markussen, S., 2007, “Use of Microbial DNA Probes as a Potential New Tool in Oil Exploration and Characterization,” SPE Paper 106418, presented at 2007 SPE International Symposium on Oilfield Chemistry, Houston, Texas, Februry 28-March 2.
Kwon, Y., 2008, “Geology of Athabasca Oil Sands in Canada,” Korean Journal of Petroleum Geology, Vol. 14, pp. 1-11.
Lazar, I. and Petrisor, I.G., 2007, “Microbial Enhanced Oil Recovery,” Petroleum Science and Technology, Vol. 25, pp. 1353-1366.
Maudgalya, S., Knapp, R.M. and McInerney, M.J., 2007, “Microbial Enhanced-Oil-Recovery Technologies: A Review of the Past, Present, and Future,” SPE Paper 106978, Proc. of SPE Production and Operations Symposium, Oklahoma city, Oklahoma, March 31 - April 3.
McInerney, M.J., Nagle, D.P. and Knapp, R.M., 2005, “Microbially Enhanced Oil Recovery: Past, Present and Future,” Petroleum Microbiology, Vol. 11, pp. 215-237.
Park, H.A., 2006, Analysis of Bacterial Community and its Correlation with Water Parameters in a Landfill Using Molecular Biological Technique, MS Thesis, Inha University.
Park, H.S., Chatterjee, I., Johnston, S., Wong, M., Thomas, R.J. and Voordouw, G., 2011, “Microbially Influenced Corrosion in Oil Sands Water Handling Facilities,” NACE 11226, Proc. of NACE International Corrosion 2011 Conference & EXPO, Houston, Texas, March 13-17.
Sayyouh, M.H., 2002, “Microbial Enhanced Oil Recovery: Research Studies in the Arabic Area During the Last Ten Years,” SPE Paper 75218, Proc. of SPE/DOE Improved Oil Recovery Symposium, Tulsa, Oklahoma, April 13-17.
Schloss, P.D., Westcott, S.L., Ryabin, T., Hall, J.R., Hartmann, E.B., Hollister, R.A., Lesniewski, B.B., Oakley, D.H., Parks, C.J., Robinson, J.W., Sahl, B., Stres, G.G., Thallinger, D.J., Van Horn. and Weber, C.F., 2009, “Introducing Mothur: Open-Source, Platform-Independent, Community- Supported Software for Describing and Comparing Microbial Communities,” Applied and Environmental Microbiology, Vol. 75, No. 23, pp. 7537-7541.
Son, H.A., Im, K.C., Lee, J.H., Kim, J.K. and Kim, H.T., 2012, “Investigation on the Trend and Field Application of MEOR Technology,” J. of the Korean Society for Geosystem Engineering, Vol. 49, No. 6, pp. 835-843.
Tringe, S.G. and Hugenholtz, P., 2008, “A Renaissance for the Pioneering 16S rRNA Gene,” Current Opinion in Microbiology, Vol. 11, No. 5, pp. 442-446.
William, K.O., Thu, N., Win, N.A. and Aung, T.K., 2011, “EEOR Success in Mann Field, Myanmar,” SPE Paper 144231, Proc. of SPE Enhanced Oil Recovery Conference, Kuala Lumpur, Malaysia, July 19-21.
Xu, T., Chen, C., Liu, C., Zhang, S., Wu, Y. and Zhang, P., 2009, “A Novel Way to Enhance the Oil Recovery Ratio by Streptococcus sp. BT-003,” J. of Basic Microbiology, Vol. 49, No. 5, pp. 477-481.
Yergeau, E., Lawrence, J.R., Sanschagrin, S., Waiser, M.J., Korber, D.R. and Greer, C.W., 2012, “Next-Generation Sequencing of Microbial Communities in the Athabasca River and Its Tributaries in Relation to Oil Sands Mining Activities,” Applied and Environmental Microbiology, Vol. 78, No. 21, pp. 7626-7637.
Youssef, N., Elshahed, M.S. and McInerney, M.J., 2009, “Microbial Processes in Oil Fields: Culprits, Problems, and Opportunities,” Advances in Applied Microbiology. Vol. 66, pp. 141-251.

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 51
No :3
Pages :404-414
DOI :https://doi.org/10.12972/ksmer.2014.51.3.404

[1] Al-Sulaimani, H., Joshi, S., Al-Wahaibi, Y., Al-Bahry, S., Elshafie, A. and Al-Bemani, A., 2011, “Microbial Biotechnology for Enhancing Oil Recovery: Current Developments and Future Prospects,” Biotechnology, Bioinformatics and Bioengineering, Vol. 1, No. 2, pp. 147-158.

[2] Aladasani, A. and Bai, B., 2010, “Recent Developments and Updated Screening Criteria of Enhanced Oil Recovery Techniques,” SPE Paper 103726, Proc. of CPS/SPE International Oil & Gas Conference and Exhibition, Beijing, China, June 8-10.

[3] Bock, M., Kampfer, P., Bosecker, K. and Dott, W., 1994, “Isolation and Characterization of Heterotrophic, Aerobic Bacteria from Oil Storage Caverns in Northern Germany,” Applied Microbiology and Biotechnology, Vol. 42, pp. 463-468.

[4] Cole, J.R., Chai, B., Farris, R.J., Wang, Q., Kulam, S.A., McGarrell, D.M., Garrity, G.M. and Tiedje, J.M., 2005, “The Ribosomal Database Project (RDP-II): Sequences and Tools for Highthroughput rRNA Analysis,” Nucleic Acids Research, Vol. 33, pp. 294-296.

[5] El Ela, M.A., El-Tayeb, S., Sayyouh, M.H., Abdel Dayem, M. and Desouky, S., 2002, “Effects of Stimulating Indigenous Bacteria in Oil Reservoirs on Relative Permeability Curves,” SPE Paper 75240, Proc. of SPE/DOE Improved Oil Recovery Symposium, Tulsa, Oklahoma, April 13-17.

[6] Harner, N.K., Richardson, T.L., Thompson, K.A., Best, R.J., Best, A.S. and Trevors, J.T., 2011, “Microbial Processes in the Athabasca Oil Sands and Their Potential Applications in Microbial Enhanced Oil Recovery,” J. of Industrial Microbiology & Biotechnology, Vol. 38, pp. 1761-1775.

[7] Haiying, C. and Weidong, W., 2007, “16S rRNA: Genes Comparative Analysis of Microbial Communities in Oil Reservoir With Nutrients Injection by Terminal Restriction Fragment Length Polymorphism Method,” SPE Paper 106398, presented at SPE Internatinoal Symposium on Oilfield Chemistry, Houston, Texas, Februry 28-March 2.

[8] Hitzman, D.O., Dennis, M. and Hitzman, D.C., 2004, “Recent Successes: MEOR Using Synergistic H2S Prevention and Increased Oil Recovery Systems,” SPE Paper 89453, Proc. of 2004 SPE/DOE Fourteenth Symposium on Improved Oil Recovery, Tulsa, Oklahoma, April 17-21.

[9] Kim, E.S., Hong, S.W. and Chung, K.S., 2011, “Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm using DGGE and Pyrosequencing,” Korean J. of Microbiology and Biotechnology, Vol. 39, No. 4, pp. 374-381.

[10] Kim, K. and Kim, D.A., 2013, “The Comparison Between Serologic Tests and Magicplex HepaTrio Real-time PCR in the Diagnosis of Viral Hepatitis,” Laboratory Medicine Online, Vol. 3, No. 1, pp. 23-28.

[11] Kotlar, H.K., Brakstad, O.G. and Markussen, S., 2007, “Use of Microbial DNA Probes as a Potential New Tool in Oil Exploration and Characterization,” SPE Paper 106418, presented at 2007 SPE International Symposium on Oilfield Chemistry, Houston, Texas, Februry 28-March 2.

[12] Kwon, Y., 2008, “Geology of Athabasca Oil Sands in Canada,” Korean Journal of Petroleum Geology, Vol. 14, pp. 1-11.

[13] Lazar, I. and Petrisor, I.G., 2007, “Microbial Enhanced Oil Recovery,” Petroleum Science and Technology, Vol. 25, pp. 1353-1366.

[14] Maudgalya, S., Knapp, R.M. and McInerney, M.J., 2007, “Microbial Enhanced-Oil-Recovery Technologies: A Review of the Past, Present, and Future,” SPE Paper 106978, Proc. of SPE Production and Operations Symposium, Oklahoma city, Oklahoma, March 31 - April 3.

[15] McInerney, M.J., Nagle, D.P. and Knapp, R.M., 2005, “Microbially Enhanced Oil Recovery: Past, Present and Future,” Petroleum Microbiology, Vol. 11, pp. 215-237.

[16] Park, H.A., 2006, Analysis of Bacterial Community and its Correlation with Water Parameters in a Landfill Using Molecular Biological Technique, MS Thesis, Inha University.

[17] Park, H.S., Chatterjee, I., Johnston, S., Wong, M., Thomas, R.J. and Voordouw, G., 2011, “Microbially Influenced Corrosion in Oil Sands Water Handling Facilities,” NACE 11226, Proc. of NACE International Corrosion 2011 Conference & EXPO, Houston, Texas, March 13-17.

[18] Sayyouh, M.H., 2002, “Microbial Enhanced Oil Recovery: Research Studies in the Arabic Area During the Last Ten Years,” SPE Paper 75218, Proc. of SPE/DOE Improved Oil Recovery Symposium, Tulsa, Oklahoma, April 13-17.

[19] Schloss, P.D., Westcott, S.L., Ryabin, T., Hall, J.R., Hartmann, E.B., Hollister, R.A., Lesniewski, B.B., Oakley, D.H., Parks, C.J., Robinson, J.W., Sahl, B., Stres, G.G., Thallinger, D.J., Van Horn. and Weber, C.F., 2009, “Introducing Mothur: Open-Source, Platform-Independent, Community- Supported Software for Describing and Comparing Microbial Communities,” Applied and Environmental Microbiology, Vol. 75, No. 23, pp. 7537-7541.

[20] Son, H.A., Im, K.C., Lee, J.H., Kim, J.K. and Kim, H.T., 2012, “Investigation on the Trend and Field Application of MEOR Technology,” J. of the Korean Society for Geosystem Engineering, Vol. 49, No. 6, pp. 835-843.

[21] Tringe, S.G. and Hugenholtz, P., 2008, “A Renaissance for the Pioneering 16S rRNA Gene,” Current Opinion in Microbiology, Vol. 11, No. 5, pp. 442-446.

[22] William, K.O., Thu, N., Win, N.A. and Aung, T.K., 2011, “EEOR Success in Mann Field, Myanmar,” SPE Paper 144231, Proc. of SPE Enhanced Oil Recovery Conference, Kuala Lumpur, Malaysia, July 19-21.

[23] Xu, T., Chen, C., Liu, C., Zhang, S., Wu, Y. and Zhang, P., 2009, “A Novel Way to Enhance the Oil Recovery Ratio by Streptococcus sp. BT-003,” J. of Basic Microbiology, Vol. 49, No. 5, pp. 477-481.

[24] Yergeau, E., Lawrence, J.R., Sanschagrin, S., Waiser, M.J., Korber, D.R. and Greer, C.W., 2012, “Next-Generation Sequencing of Microbial Communities in the Athabasca River and Its Tributaries in Relation to Oil Sands Mining Activities,” Applied and Environmental Microbiology, Vol. 78, No. 21, pp. 7626-7637.

[25] Youssef, N., Elshahed, M.S. and McInerney, M.J., 2009, “Microbial Processes in Oil Fields: Culprits, Problems, and Opportunities,” Advances in Applied Microbiology. Vol. 66, pp. 141-251.

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