Assessment of Metal Immobilization and Bioavailability Affected by Bacteria in Soil

doi:10.12972/ksmer.2016.53.5.431

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2016 Vol.53, Issue 5 Preview Page Next Page

Research Paper (Special Issue)

Assessment of Metal Immobilization and Bioavailability Affected by Bacteria in Soil 세균처리에 의한 금속의 토양 내 고정화 효과 및 생물 이용도 평가: 박진희
Jin Hee Park

31 October 2016. pp. 431-439

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Abstract

Bacteria that adsorb Cd were isolated from metal contaminated soil and identified as Exiguobacteriumspecies. The effect of the isolated bacteria on metal immobilization was evaluated by pot experiment using heavy metal contaminated soils collected from abandoned mine sites. The Exiguobacterium sp. reduced the uptake of Cd by 30% in lettuce leaves, and Pb and Zn by 61% and 54% in lettuce roots, respectively, in the case of extremely high metal concentrations in soil. Bacteria decreased the mobile heavy metal contents and increased the organic bound metal fraction in bulk soil. In the case of extremely high soil metal concentration, bacterial treatment did not lead to significant differences in the heavy metal speciation within the soil. Nevertheless, immobilization of heavy metals by biosorption in the soil can facilitate the establishment of vegetation in mine soil, thereby mitigating the potential risk of distribution and leaching of heavy metals.

Keywords

Biosorption

Bacteria

Heavy metal

Immobilization

Mine soil

중금속으로 오염된 토양에서 분리된 카드뮴을 흡착할 수 있는 세균인 Exiguobacterium sp.를 이용하여 폐광지역에서 채취한 중금속 오염 토양에서 세균에 의한 중금속의 고정화를 식물 생육 실험을 통해 평가하였다.Exiguobacterium sp.를 토양에 처리했을 때 오염 정도가 심한 토양에서 세균은 상추 잎의 카드뮴 함량을 약 30% 감소시켰으며 납과 아연의 함량을 뿌리에서 각각 61%, 54% 감소시켰다. 연속 추출 결과 세균 처리는 벌크토양에서 이동도가 높은 중금속의 함량을 감소시켰으며 유기물과 결합된 형태의 중금속 함량을 증가시켰다. 그러나 그 결과는 토양의 총 중금속 함량에 따라 다르게 나타났으며 오염 정도가 심한 토양에서 세균의 처리는 토양 내 중금속의 존재 형태에 크게 영향을 미치지 않았다. 그럼에도 불구하고 토양에서 생물흡착에 의한 중금속의 고정화는 광산 토양에서 식생 형성을 가능하게 하여 중금속의 분산이나 용출의 잠재적인 위험을 완화시킬 수 있다.

키워드

생물흡착

세균

중금속

고정화

광산 토양

References

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Becker, A., Klock, W., Friese, K., Schreck, P., Treutler, H.C., Spettel, B. and Duff, M.C., 2001, “Lake Süßer see as anatural sink for heavy metals from copper mining,” Journal of Geochemical Exploration, Vol. 74, No. 1-3, pp. 205-217.
Beesley, L., Moreno-Jiménez, E.M. and Gomez-Eyles, J.L., 2010, “Effects of biochar and greenwaste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil,” Environmental Pollution, Vol. 158, No. 6, pp. 2282-2287.
Belimov, A.A., Hontzeas, N., Safronova, V.I., Demchinskaya, S.V., Piluzza, G., Bullitta, S. and Glick, B.R., 2005, “Cadmium- tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.),” Soil Biology and Biochemistry, Vol. 37, No. 2, pp. 241-250.
Bolan, N., Kunhikrishnan, A., Thangarajan, R., Kumpiene, J., Park, J., Makino, T. and Kirkham, M.B., 2014, “Remediation of heavy metal(loid)s contaminated soils-To mobilize or to immobilize?,” Journal of Hazardous Materials, Vol. 266, pp. 141-166.
Chander, K. and Joergensen, R.G., 2002, “Decomposition of 14C labeled glucose in a Pb-contaminated soil remediated with synthetic zeolite and other amendments,” Soil Biology and Biochemistry, Vol. 34, No. 5, pp. 643-649.
Cui, Y., Dong, Y., Li, H. and Want, Q., 2004, “Effect of elemental sulphur on solubility of soil heavy metals and their uptake by maize,” Environmental International, Vol. 30, No. 3, pp. 323-328.
Garate, A., Ramos, I., Manzanares, M. and Lucena J.J, 1993, “Cadmium uptake and distribution in three cultivars of Lactuca sp.,” Environmental Contamination and Toxicology, Vol. 50, pp. 709-716.
Illera, V., Walter, I., Souza, P. and Cala, V., 2000, “Short effects of biosolid and municipal solid waste applications on heavy metals distribution in a degraded soil under a semi- arid environment,” Science of The Total Environment, Vol. 255, No. 1-3, pp. 29-44.
Jones Jr, J.B., 2001, Laboratory Guide for Conducting Soil Tests and Plant Analysis, CRC Press, Boca Raton, USA.
Kabata-Pendias, A., 1992, Trace Elements in Soil and Plant, second ed. CRC Press, London, UK.
Ko, M.S., Park, H.S., Kim, K.W. and Lee, J.U., 2010, “Experimental study on stabilization of heavy metals by biofilm developed on soil particle surface,” Journal of Korean Society of Mineral and Energy Resources Engineers, Vol. 47, No. 2, pp. 158-167.
Kopittke, P.M., Asher, C.J., Blamey, FlP.C., Auchterlonie, G.J., Guo, Y.N. and Menzies, N.W., 2008, “Localization and chemical speciation of Pb in roots of signal grass (Brachiaria decumbens) and Rhodes grass (Chloris gayana),” Environmental Science and Technology, Vol. 42, No. 12, pp. 4595-4599.
Korean Ministry of Environment (2014) Soil conservation law.
Kuffner, M., De Maria, S., Puschenreiter, M., Fallmann, K., Wieshammer, G., Gorfer, M., Strauss, J., Rivelli, A.R. and Sessitsch, A., 2010, “Culturable bacteria from Zn- and Cd-accumulating Saliz caprea with differential effects on plant growth and heavy metal availability,” Journal of Applied Microbiology, Vol. 108, No. 4, pp. 1471-1484.
Kumar, K.S., Dahms, H.U., Won, E.J., Lee, J.S. and Shin, K.H., 2015, “Microalgae-A promising tool for heavy metal remediation,” Ecotoxicology and Environmental Safety, Vol. 113, pp. 329-352.
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Lovley, D.R. and Coates, J.D., 1997, “Bioremediation of metal contamination,” Current Opinion in Biotechnology, Vol. 8, No. 3, pp. 285-289.
Luo, L.C., Shen, Z.G. and Li, X.D., 2008, “Root exudates increase metal accumulation mixed cultures: Implications for naturally enhanced phytoextraction,” Water, Air and Soil Pollution, Vol. 193, No. 1, pp. 147-154.
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Park, J.H., Lamb, D., Paneerselvam, P., Choppala, G., Bolan, N. and Chung, J.W., 2011, “Role of organic amendments on enhanced bioremediation of heavy metal(loid) contaminated soils,” Journal of Hazardous Materials, Vol. 185, No. 2-3, pp. 549-574.
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Rajkumar, M., Ae, N., Prasad, M.N.V. and Freitas, H., 2010, “Potential of siderophore-producing bacteria for improving heavy metal phytoextraction,” Trends in Biotechnology, Vol. 28, No. 3, pp. 142-149.
Sager, M., Park, J.H. and Chon, H.T., 2007, “The effect of soil bacteria and perlite on plant growth and soil properties in metal contaminated samples,” Water Air Soil Pollution, Vol. 179, No. 1, pp. 265-281.
Selim, H.M., Ma, L., Zhu, H., Amacher, M.C. and Iskandar, I.K., 1995, Adsorption-desorption and transport of copper in soils, in: INRA (Eds.), Contaminated Soils, Insitut National de la Recherche Agronomique, France, pp. 223-232.
Shi, W.Y., Shao H.B., Li, H., Shao, MA. and Du, S., 2009, “Progress in the remediation of hazardous heavy metal- polluted soils by natural zeolite,” Journal of Hazardous Materials, Vol. 170, No. 1, pp. 1-6.
Tao, S., Liu, W.X., Chen, Y.J., Xu, F.L., Dawson, R.W. and Li, B.G., 2004, “Evaluation of factors influence root-induced changes of copper fractionation in rhizosphere of a calcareous soil,” Environmental Pollution, Vol. 129, No. 1, pp. 5-12.
Tessier, A., Campbell, P.G.C. and Bisson, M., 1979, “Sequential extraction procedure for the speciation of particulate trace metals,” Analytical Chemistry, Vol. 51, No. 7, pp. 844-851.
Ullah, A., Heng, S., Munis, M.F.H., Fahad S. and Yang, X., 2015, “Phytoremediation of heavy metals assisted by plant growth promoting (PGP) bacteria: A review,” Environmental and Experimental Botany, Vol. 117, pp. 28-40.
Uraguchi, S., Mori, S., Kuramata, M., Kawasaki, A., Ara, T. and Ishikawa, S., 2009, “Root-to-shoot Cd translocation via the xylem is the major process determining shoot andgrain cadmium accumulation in rice,” Journal of Experimental Botany, Vol. 60, No. 9, pp. 2677-2688.
Vázquez, S. and Carpena-Ruiz, R., 2005, “Use of perlite in cadmium plant studies: an approach to polluted soil conditions,” Journal of Environmental Monitoring, Vol. 7, No. 12, pp. 1355-1358.
Wang, J. and Chen, C., 2009, “Biosorbents for heavy metals removal and their future,” Biotechnology Advances, Vol. 27, No. 2, pp. 195-226.
Wu, S.C., Luo, Y.M., Cheung, K.C. and Wong, M.H., 2006, “Influence of bacteria on Pb and Zn speciation, mobility and bioavailability in soil: A laboratory study,” Environmental Pollution, Vol. 144, No. 3, pp. 765-773.

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 53
No :5
Pages :431-439
DOI :https://doi.org/10.12972/ksmer.2016.53.5.431

[1] Achiba, W.B., Gabteni, N., Lakhdar, A., Laing, G.D., Verloo, M., Jedidi, N. and Gallali, T., 2009, “Effects of 5-year application of municipal solid waste compost on the distribution and mobility of heavy metals in a Tunisian calcareous soil,” Agriculture, Ecosystems and Environment, Vol. 130, No. 3-4, pp. 156-163.

[2] Becker, A., Klock, W., Friese, K., Schreck, P., Treutler, H.C., Spettel, B. and Duff, M.C., 2001, “Lake Süßer see as anatural sink for heavy metals from copper mining,” Journal of Geochemical Exploration, Vol. 74, No. 1-3, pp. 205-217.

[3] Beesley, L., Moreno-Jiménez, E.M. and Gomez-Eyles, J.L., 2010, “Effects of biochar and greenwaste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil,” Environmental Pollution, Vol. 158, No. 6, pp. 2282-2287.

[4] Belimov, A.A., Hontzeas, N., Safronova, V.I., Demchinskaya, S.V., Piluzza, G., Bullitta, S. and Glick, B.R., 2005, “Cadmium- tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.),” Soil Biology and Biochemistry, Vol. 37, No. 2, pp. 241-250.

[5] Bolan, N., Kunhikrishnan, A., Thangarajan, R., Kumpiene, J., Park, J., Makino, T. and Kirkham, M.B., 2014, “Remediation of heavy metal(loid)s contaminated soils-To mobilize or to immobilize?,” Journal of Hazardous Materials, Vol. 266, pp. 141-166.

[6] Chander, K. and Joergensen, R.G., 2002, “Decomposition of 14C labeled glucose in a Pb-contaminated soil remediated with synthetic zeolite and other amendments,” Soil Biology and Biochemistry, Vol. 34, No. 5, pp. 643-649.

[7] Cui, Y., Dong, Y., Li, H. and Want, Q., 2004, “Effect of elemental sulphur on solubility of soil heavy metals and their uptake by maize,” Environmental International, Vol. 30, No. 3, pp. 323-328.

[8] Garate, A., Ramos, I., Manzanares, M. and Lucena J.J, 1993, “Cadmium uptake and distribution in three cultivars of Lactuca sp.,” Environmental Contamination and Toxicology, Vol. 50, pp. 709-716.

[9] Illera, V., Walter, I., Souza, P. and Cala, V., 2000, “Short effects of biosolid and municipal solid waste applications on heavy metals distribution in a degraded soil under a semi- arid environment,” Science of The Total Environment, Vol. 255, No. 1-3, pp. 29-44.

[10] Jones Jr, J.B., 2001, Laboratory Guide for Conducting Soil Tests and Plant Analysis, CRC Press, Boca Raton, USA.

[11] Kabata-Pendias, A., 1992, Trace Elements in Soil and Plant, second ed. CRC Press, London, UK.

[12] Ko, M.S., Park, H.S., Kim, K.W. and Lee, J.U., 2010, “Experimental study on stabilization of heavy metals by biofilm developed on soil particle surface,” Journal of Korean Society of Mineral and Energy Resources Engineers, Vol. 47, No. 2, pp. 158-167.

[13] Kopittke, P.M., Asher, C.J., Blamey, FlP.C., Auchterlonie, G.J., Guo, Y.N. and Menzies, N.W., 2008, “Localization and chemical speciation of Pb in roots of signal grass (Brachiaria decumbens) and Rhodes grass (Chloris gayana),” Environmental Science and Technology, Vol. 42, No. 12, pp. 4595-4599.

[14] Korean Ministry of Environment (2014) Soil conservation law.

[15] Kuffner, M., De Maria, S., Puschenreiter, M., Fallmann, K., Wieshammer, G., Gorfer, M., Strauss, J., Rivelli, A.R. and Sessitsch, A., 2010, “Culturable bacteria from Zn- and Cd-accumulating Saliz caprea with differential effects on plant growth and heavy metal availability,” Journal of Applied Microbiology, Vol. 108, No. 4, pp. 1471-1484.

[16] Kumar, K.S., Dahms, H.U., Won, E.J., Lee, J.S. and Shin, K.H., 2015, “Microalgae-A promising tool for heavy metal remediation,” Ecotoxicology and Environmental Safety, Vol. 113, pp. 329-352.

[17] Liu, D., Jiang, W., Liu, C., Xin, C. and Hou, W., 2000, “Uptake and accumulation of lead by roots, hypocotyls and shoots of Indian mustard [Brassica juncea (L.)],” Bioresource Technology, Vol. 71, No. 3, pp. 273-277.

[18] Lovley, D.R. and Coates, J.D., 1997, “Bioremediation of metal contamination,” Current Opinion in Biotechnology, Vol. 8, No. 3, pp. 285-289.

[19] Luo, L.C., Shen, Z.G. and Li, X.D., 2008, “Root exudates increase metal accumulation mixed cultures: Implications for naturally enhanced phytoextraction,” Water, Air and Soil Pollution, Vol. 193, No. 1, pp. 147-154.

[20] Madhaiyan, M., Poonguzhali, S. and Sa, T., 2007, “Metal tolerating methylotrophic bacteria reduces nickel and cadmium toxicity and promotes plant growth of tomato (Lycopersicon esculentum L.),” Chemosphere, Vol. 69, No. 2, pp. 220-228.

[21] Park, J.H. and Chon, H.T., 2016, “Characterization of cadmium biosorption by Exiguobacterium sp. isolated from farmland soil near Cu-Pb-Zn mine,” Environmental Science and Pollution Research, Vol. 23, No. 2, pp. 11814-11822.

[22] Park, J.H., Lamb, D., Paneerselvam, P., Choppala, G., Bolan, N. and Chung, J.W., 2011, “Role of organic amendments on enhanced bioremediation of heavy metal(loid) contaminated soils,” Journal of Hazardous Materials, Vol. 185, No. 2-3, pp. 549-574.

[23] Perez, D.V., Alcantra, S., Ribeiro, C.C., Pereira, R.E., Fontes, G.C., Wasserman, M.A., Venezuela,T.C., Meneguelli, N.A. and Parradas, C.A.A., 2007, “Composted municipal waste effects on chemical properties of Brazilian soil,” Bioresource Technology, Vol. 98, No. 3, pp. 525-533.

[24] Rajkumar, M., Ae, N., Prasad, M.N.V. and Freitas, H., 2010, “Potential of siderophore-producing bacteria for improving heavy metal phytoextraction,” Trends in Biotechnology, Vol. 28, No. 3, pp. 142-149.

[25] Sager, M., Park, J.H. and Chon, H.T., 2007, “The effect of soil bacteria and perlite on plant growth and soil properties in metal contaminated samples,” Water Air Soil Pollution, Vol. 179, No. 1, pp. 265-281.

[26] Selim, H.M., Ma, L., Zhu, H., Amacher, M.C. and Iskandar, I.K., 1995, Adsorption-desorption and transport of copper in soils, in: INRA (Eds.), Contaminated Soils, Insitut National de la Recherche Agronomique, France, pp. 223-232.

[27] Shi, W.Y., Shao H.B., Li, H., Shao, MA. and Du, S., 2009, “Progress in the remediation of hazardous heavy metal- polluted soils by natural zeolite,” Journal of Hazardous Materials, Vol. 170, No. 1, pp. 1-6.

[28] Tao, S., Liu, W.X., Chen, Y.J., Xu, F.L., Dawson, R.W. and Li, B.G., 2004, “Evaluation of factors influence root-induced changes of copper fractionation in rhizosphere of a calcareous soil,” Environmental Pollution, Vol. 129, No. 1, pp. 5-12.

[29] Tessier, A., Campbell, P.G.C. and Bisson, M., 1979, “Sequential extraction procedure for the speciation of particulate trace metals,” Analytical Chemistry, Vol. 51, No. 7, pp. 844-851.

[30] Ullah, A., Heng, S., Munis, M.F.H., Fahad S. and Yang, X., 2015, “Phytoremediation of heavy metals assisted by plant growth promoting (PGP) bacteria: A review,” Environmental and Experimental Botany, Vol. 117, pp. 28-40.

[31] Uraguchi, S., Mori, S., Kuramata, M., Kawasaki, A., Ara, T. and Ishikawa, S., 2009, “Root-to-shoot Cd translocation via the xylem is the major process determining shoot andgrain cadmium accumulation in rice,” Journal of Experimental Botany, Vol. 60, No. 9, pp. 2677-2688.

[32] Vázquez, S. and Carpena-Ruiz, R., 2005, “Use of perlite in cadmium plant studies: an approach to polluted soil conditions,” Journal of Environmental Monitoring, Vol. 7, No. 12, pp. 1355-1358.

[33] Wang, J. and Chen, C., 2009, “Biosorbents for heavy metals removal and their future,” Biotechnology Advances, Vol. 27, No. 2, pp. 195-226.

[34] Wu, S.C., Luo, Y.M., Cheung, K.C. and Wong, M.H., 2006, “Influence of bacteria on Pb and Zn speciation, mobility and bioavailability in soil: A laboratory study,” Environmental Pollution, Vol. 144, No. 3, pp. 765-773.

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