A Study on Simultaneous Bioleaching of Copper and Manganese from Boleo Low-Grade Copper Oxide Ore Using Sulfur-Oxidizing Bacterium Acidithiobacillus thiooxidans

doi:10.12972/ksmer.2016.53.5.420

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2016 Vol.53, Issue 5 Preview Page Next Page

Research Paper (Special Issue)

A Study on Simultaneous Bioleaching of Copper and Manganese from Boleo Low-Grade Copper Oxide Ore Using Sulfur-Oxidizing Bacterium Acidithiobacillus thiooxidans 황산화균 Acidithiobacillus thiooxidans를 이용한 Boleo 저품위 산화동광 내 구리 및 망간의 동시추출 연구: 유지용·한협조·이종운
Ji-Yong Yu, Hyeop-Jo Han and Jong-Un Lee

31 October 2016. pp. 420-430

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Abstract

Simultaneous bioleaching of Cu and Mn from Boleo low-grade Cu oxide ore was investigated usinga sulfur-oxidizing bacterium Acidithiobacillus thiooxidans. The amounts of Cu and Mn were 2~3% in the oreas phosphates, silicates, and oxides. A. thiooxidans leached Cu with less amount of Mn in fresh culture medium Increase of inoculated bacterial cells or addition of yeast extract to the culture medium increased the amounts of Cu and Mn extracted from the ore. Extracted Mn existed dominantly as colloidal suspensions rather than dissolved phase. When bacterial culture incubated for one month was applied to the ore with medium, the lowest pH appeared and the extraction efficiency of dissolved Cu and Mn was significantly enhanced. Such enhancement was attributed to bacterial metabolism instead of chemical composition of culture medium itself. The results indicated that bioleaching may be efficiently applied to lixiviate Cu and Mn simultaneously from Boleo Cu oxide ore.

Keywords

Bioleaching

Boleo

Copper oxide ore

Copper

Manganese

황산화균인 Acidithiobacillus thiooxidans를 이용한 미생물학적 용출법을 적용시켜 Boleo 저품위 산화동광 내 구리와 망간의 동시추출이 가능한지 확인하였다. 산화동광 내 구리와 망간은 각 2~3% 존재하며 광물형태는 주로 인산염, 규산염, 산화광물 형태였다. 신선한 배양액 내에서 A. thiooxidans는 산화동광에서 구리를 용출시켰으나 망간 용출량은 낮았다. 투입하는 미생물 수를 증가시키거나 효모추출물을 첨가한 결과 구리와 망간 용출량이 다소 증가하였으며 효모추출물 첨가 시 높은 효율을 보였다. 용출된 망간은 용존 상태보다 콜로이드 상태로 존재하였다. A. thiooxidans를 한 달간 배양한 배양액을 산화동광에 적용한 결과 가장 낮은 pH를 보였으며 구리뿐만 아니라 용존 상태 망간의 용출효율도 대폭 증가하였다. 이 효율 증진은 배양액의 화학적 조성보다 배양액 내 A. thiooxidans의 대사작용에 기인하였다. 이러한 결과는 Boleo의 저품위 산화동광으로부터 구리 및 망간을 동시 추출하는 데 있어 미생물학적 용출법이 적용 가능함을 나타내는 것이다.

키워드

미생물학적 용출

Boleo

산화동광

구리

망간

References

Abdollahi, H., Noaparast, M., Shafaei, S.Z., Manafi, Z., Muñoz, J.A. and Tuovinen, O.H., 2015, “Silver-catalyzed bioleaching of copper, molybdenum and rhenium from a chalcopyrite-molybdenite concentrate,” Int. Biodeter. Biodegra., Vol. 104, pp. 194-200.
Anand, S., Das, S.C., Das, R.P. and Jena, P.K., 1988, “Leaching of manganese nodules at elevated temperature and pressure in the presence of oxygen,” Hydrometallurgy, Vol. 20, No. 2, pp.155-168.
Arena, F.A., Suegama, P.H., Bevilaqua, D., dos Santos, A.L., Fugivara, C.S. and Benedetti, A.V., 2016, “Simulating the main stages of chalcopyrite leaching and bioleaching in ferrous ions solution: An electrochemical impedance study with a modified carbon paste electrode,” Miner. Eng., Vol. 92, pp. 229-241.
Bafghi, M.S., Zakeri, A., Ghasemi, Z. and Adeli, M., 2008, “Reductive dissolution of manganese ore in sulfuric acid in the presence of iron metal,” Hydrometallurgy, Vol. 90, No. 2, pp. 207-212.
Brierley, C.L. and Brierley, J.A., 1973, “A chemoautotrophic and thermophilic microorganism isolated from an acid hot spring,” Can. J. Microbiol., Vol. 19, No. 2, pp. 183-188.
Choi, S.-G., Kim, C.S., Ko, E.-M., Kim, S.-Y. and Jo, H.Y., 2008, “Mineral economic index and comprehensive demand prediction for strategic minerals: Copper, zinc, lead, and nickel,” Econ. Environ. Geol., Vol. 41, No. 3, pp. 345-357.
Crundwell, F.K., 2013, “The dissolution and leaching of minerals: mechanisms, myths and misunderstandings,” Hydrometallurgy, Vol. 139, pp. 132-148.
Dan, Z., Zhang, Y., Cai, J., Li, X., Duan, N. and Xin, B., 2016, “Reductive leaching of manganese from manganese dioxide ores by bacterial-catalyzed two-ores method,” Int. J. Miner. Processing, Vol. 150, pp. 24-31.
Dong, Y.B., Lin, H., Zhou, S., Xu, X. and Zhang, Y., 2013, “Effects of quartz addition on chalcopyrite bioleaching in shaking flasks,” Miner. Eng., Vol. 46, pp. 177-179.
Fang, D. and Zhou, L.X., 2006, “Effect of sludge dissolved organic matter on oxidation of ferrous iron and sulfur by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxi- dans,” Water, Air, and Soil Pollution, Vol. 171, No. 1, pp. 81-94.
Feng, Y.L., Zhang, S.Y. and Li, H.R., 2016, “Reductive leaching of manganese from low-grade pyrolusite ore in sulfuric acid using pyrolysis-pretreated sawdust as a reductant,” Int. J. Minerals, Metallurgy, and Materials, Vol. 23, No. 3, pp. 241-246.
Gu, X. and Wong, J.W., 2004, “Identification of inhibitory substances affecting bioleaching of heavy metals from anaerobically digested sewage sludge,” Environ. Sci. Technol., Vol. 38, No. 10, pp. 2934-2939.
Hackl, R.P., Dreisinger, D.B., Peters, E. and King, J.A., 1995, “Passivation of chalcopyrite during oxidative leaching in sulfate media,” Hydrometallurgy, Vol. 39, No. 1, pp. 25-48.
Han, K.S., 2016, Reductive Leaching of Copper Ore from El Boleo Mine, MSc Thesis, Chonnam National University, Korea, p. 50.
Hocheng, H., Su, C. and Jadhav, U., 2014, “Bioleaching of metals from steel slag by Acidithiobacillus thiooxidans culture supernatant,” Chemosphere, Vol. 117, pp. 652-657.
Holmes, P.R. and Crundwell, F.K., 2013, “Polysulfides do not cause passivation: results from the dissolution of pyrite and implication for other sulfide minerals,” Hydrometallurgy, Vol. 139, pp. 101-110.
Johnson, D.B., 2013, “Development and application of biotechnologies in the metal mining industry,” Environ. Sci. Pollut. Res., Vol. 20, No. 11, pp. 7768-7776.
Khoshkhoo, M., Dopson, M., Shchukarev, A. and Sandström, Å, 2014, “Chalcopyrite leaching and bioleaching: an X-ray photoelectron spectroscopic (XPS) investigation on the nature of hindered dissolution,” Hydrometallurgy, Vol. 149, pp. 220-227.
Konishi, Y., Yoshida, S. and Asai, S., 1998, “Effect of yeast extract supplementation in leach solution on bioleaching rate of pyrite by acidophilic thermophile Acidianus brierleyi,” Biotechnol. Bioeng., Vol. 58, No. 6, pp. 663-667.
Lara, R., García-Meza, J.V., González, I. and Cruz, R., 2013, “Influence of the surface speciation on biofilm attachment to chalcopyrite by Acidithiobacillus thiooxidans,” Appl. Microbiol. Biotechnol., Vol. 97, No. 6, pp. 2711- 2724.
Lee, E.Y., Noh, S.R., Cho, K.S. and Ryu, H.W., 2001, “Leaching of Mn, Co, and Ni from manganese nodules using an anaerobic bioleaching method,” J. Biosci. Bioeng., Vol. 92, No. 4, pp. 354-359.
Liu, M., Wen, J., Tan, G., Liu, G. and Wu, B., 2016, “Experimental studies and pilot plants tests for acid leaching of low-grade copper oxide ores at the Tuwu Copper Mine,” Hydrometallurgy, Vol. 165, Part 2, pp. 227-232.
Lovley, D.R., 1991, “Dissimilatory Fe(III) and Mn(IV) reduction,” Microbiol. Rev., Vol. 55, No. 2, pp. 259-287.
Mehta, K.D., Das, C. and Pandey, B.D., 2010, “Leaching of copper, nickel and cobalt from Indian Ocean manganese nodules by Aspergillus niger,” Hydrometallurgy, Vol. 105, No. 1, pp. 89-95.
Norris, P.R. and Barr, D.W., 1985, “Growth and iron oxidation by acidophilic moderate thermophiles,” FEMS Microbiol. Lett., Vol. 28, No. 3, pp. 221-224.
Paixdo, J.M.M., Amaral, J.C., Memoria, L.E. and Freitas, L.R., 1995, “Sulphation of Carajás manganese ore,” Hydrometallurgy, Vol. 39, No. 1, pp. 215-222.
Park, C.-Y. and Kim, B.-J., 2010, “The change of isoelectric points and the attachment for chalcopyrite by indigenous bacteria,” J. of Mineral and Energy Resources., Vol. 47, No. 6, pp. 823-833.
Park, C.-Y., Kim, S.-O. and Kim, B.-J., 2010, “The characteristic of selective attachment and bioleaching for pyrite using indigenous acidophilic bacteria at 42°C,” Econ. Environ. Geol., Vol. 43, No. 2, pp. 109-121.
Park, J.S., 2016, A Novel Process for Manufacturing the Electrolyte for Electrowinning EMM (Eletrolytic Manganese Metal) from El Boleo Mine Waste Water, MSc Thesis, Chonnam National University, Korea, p. 60.
Qin, W., Yang, C., Lai, S., Wang, J., Liu, K. and Zhang, B., 2013, “Bioleaching of chalcopyrite by moderately thermophilic microorganisms,” Bioresour. Technol., Vol. 129, pp. 200-208.
Rohwerder, T., Gehrke, T., Kinzler, K. and Sand, W., 2003, “Bioleaching review part A: Progress in bioleaching: fundamentals and mechanisms of bacterial metal sulfide oxidation,” Appl. Microbiol. Biotechnol., Vol. 63, pp. 239-248.
Salas, R.E.D.R., 2011, Metallogenesis for the Boleo and Cananea Copper Mining Districts: A Contribution to the Understanding of Copper Ore Deposits in Northwestern Mexico, Ph.D. Thesis, The University of Arizona, USA, p. 226.
Sandström, A., Shchukarev, A. and Paul, J., 2005, “XPS characterisation of chalcopyrite chemically and bio-leached at high and low redox potential,” Miner. Eng., Vol. 18, No. 5, pp. 505-515.
Sun, W.Y., Su, S.J., Wang, Q.Y. and Ding, S.L., 2013, “Lab-scale circulation process of electrolytic manganese production with low-grade pyrolusite leaching by SO₂,” Hydrometallurgy, Vol. 133, pp. 118-125.
Vavra, J.P. and Frederick, L.R., 1952, “The effect of sulfur oxidation on the availability of manganese,” Soil Science Society of America Journal, Vol. 16, No. 2, pp. 141-144.
Zhang, W. and Cheng, C.Y., 2007, “Manganese metallurgy review. Part I: Leaching of ores/secondary materials and recovery of electrolytic/chemical manganese dioxide,” Hydrometallurgy, Vol. 89, No. 3, pp. 137-159.

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 53
No :5
Pages :420-430
DOI :https://doi.org/10.12972/ksmer.2016.53.5.420

[1] Abdollahi, H., Noaparast, M., Shafaei, S.Z., Manafi, Z., Muñoz, J.A. and Tuovinen, O.H., 2015, “Silver-catalyzed bioleaching of copper, molybdenum and rhenium from a chalcopyrite-molybdenite concentrate,” Int. Biodeter. Biodegra., Vol. 104, pp. 194-200.

[2] Anand, S., Das, S.C., Das, R.P. and Jena, P.K., 1988, “Leaching of manganese nodules at elevated temperature and pressure in the presence of oxygen,” Hydrometallurgy, Vol. 20, No. 2, pp.155-168.

[3] Arena, F.A., Suegama, P.H., Bevilaqua, D., dos Santos, A.L., Fugivara, C.S. and Benedetti, A.V., 2016, “Simulating the main stages of chalcopyrite leaching and bioleaching in ferrous ions solution: An electrochemical impedance study with a modified carbon paste electrode,” Miner. Eng., Vol. 92, pp. 229-241.

[4] Bafghi, M.S., Zakeri, A., Ghasemi, Z. and Adeli, M., 2008, “Reductive dissolution of manganese ore in sulfuric acid in the presence of iron metal,” Hydrometallurgy, Vol. 90, No. 2, pp. 207-212.

[5] Brierley, C.L. and Brierley, J.A., 1973, “A chemoautotrophic and thermophilic microorganism isolated from an acid hot spring,” Can. J. Microbiol., Vol. 19, No. 2, pp. 183-188.

[6] Choi, S.-G., Kim, C.S., Ko, E.-M., Kim, S.-Y. and Jo, H.Y., 2008, “Mineral economic index and comprehensive demand prediction for strategic minerals: Copper, zinc, lead, and nickel,” Econ. Environ. Geol., Vol. 41, No. 3, pp. 345-357.

[7] Crundwell, F.K., 2013, “The dissolution and leaching of minerals: mechanisms, myths and misunderstandings,” Hydrometallurgy, Vol. 139, pp. 132-148.

[8] Dan, Z., Zhang, Y., Cai, J., Li, X., Duan, N. and Xin, B., 2016, “Reductive leaching of manganese from manganese dioxide ores by bacterial-catalyzed two-ores method,” Int. J. Miner. Processing, Vol. 150, pp. 24-31.

[9] Dong, Y.B., Lin, H., Zhou, S., Xu, X. and Zhang, Y., 2013, “Effects of quartz addition on chalcopyrite bioleaching in shaking flasks,” Miner. Eng., Vol. 46, pp. 177-179.

[10] Fang, D. and Zhou, L.X., 2006, “Effect of sludge dissolved organic matter on oxidation of ferrous iron and sulfur by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxi- dans,” Water, Air, and Soil Pollution, Vol. 171, No. 1, pp. 81-94.

[11] Feng, Y.L., Zhang, S.Y. and Li, H.R., 2016, “Reductive leaching of manganese from low-grade pyrolusite ore in sulfuric acid using pyrolysis-pretreated sawdust as a reductant,” Int. J. Minerals, Metallurgy, and Materials, Vol. 23, No. 3, pp. 241-246.

[12] Gu, X. and Wong, J.W., 2004, “Identification of inhibitory substances affecting bioleaching of heavy metals from anaerobically digested sewage sludge,” Environ. Sci. Technol., Vol. 38, No. 10, pp. 2934-2939.

[13] Hackl, R.P., Dreisinger, D.B., Peters, E. and King, J.A., 1995, “Passivation of chalcopyrite during oxidative leaching in sulfate media,” Hydrometallurgy, Vol. 39, No. 1, pp. 25-48.

[14] Han, K.S., 2016, Reductive Leaching of Copper Ore from El Boleo Mine, MSc Thesis, Chonnam National University, Korea, p. 50.

[15] Hocheng, H., Su, C. and Jadhav, U., 2014, “Bioleaching of metals from steel slag by Acidithiobacillus thiooxidans culture supernatant,” Chemosphere, Vol. 117, pp. 652-657.

[16] Holmes, P.R. and Crundwell, F.K., 2013, “Polysulfides do not cause passivation: results from the dissolution of pyrite and implication for other sulfide minerals,” Hydrometallurgy, Vol. 139, pp. 101-110.

[17] Johnson, D.B., 2013, “Development and application of biotechnologies in the metal mining industry,” Environ. Sci. Pollut. Res., Vol. 20, No. 11, pp. 7768-7776.

[18] Khoshkhoo, M., Dopson, M., Shchukarev, A. and Sandström, Å, 2014, “Chalcopyrite leaching and bioleaching: an X-ray photoelectron spectroscopic (XPS) investigation on the nature of hindered dissolution,” Hydrometallurgy, Vol. 149, pp. 220-227.

[19] Konishi, Y., Yoshida, S. and Asai, S., 1998, “Effect of yeast extract supplementation in leach solution on bioleaching rate of pyrite by acidophilic thermophile Acidianus brierleyi,” Biotechnol. Bioeng., Vol. 58, No. 6, pp. 663-667.

[20] Lara, R., García-Meza, J.V., González, I. and Cruz, R., 2013, “Influence of the surface speciation on biofilm attachment to chalcopyrite by Acidithiobacillus thiooxidans,” Appl. Microbiol. Biotechnol., Vol. 97, No. 6, pp. 2711- 2724.

[21] Lee, E.Y., Noh, S.R., Cho, K.S. and Ryu, H.W., 2001, “Leaching of Mn, Co, and Ni from manganese nodules using an anaerobic bioleaching method,” J. Biosci. Bioeng., Vol. 92, No. 4, pp. 354-359.

[22] Liu, M., Wen, J., Tan, G., Liu, G. and Wu, B., 2016, “Experimental studies and pilot plants tests for acid leaching of low-grade copper oxide ores at the Tuwu Copper Mine,” Hydrometallurgy, Vol. 165, Part 2, pp. 227-232.

[23] Lovley, D.R., 1991, “Dissimilatory Fe(III) and Mn(IV) reduction,” Microbiol. Rev., Vol. 55, No. 2, pp. 259-287.

[24] Mehta, K.D., Das, C. and Pandey, B.D., 2010, “Leaching of copper, nickel and cobalt from Indian Ocean manganese nodules by Aspergillus niger,” Hydrometallurgy, Vol. 105, No. 1, pp. 89-95.

[25] Norris, P.R. and Barr, D.W., 1985, “Growth and iron oxidation by acidophilic moderate thermophiles,” FEMS Microbiol. Lett., Vol. 28, No. 3, pp. 221-224.

[26] Paixdo, J.M.M., Amaral, J.C., Memoria, L.E. and Freitas, L.R., 1995, “Sulphation of Carajás manganese ore,” Hydrometallurgy, Vol. 39, No. 1, pp. 215-222.

[27] Park, C.-Y. and Kim, B.-J., 2010, “The change of isoelectric points and the attachment for chalcopyrite by indigenous bacteria,” J. of Mineral and Energy Resources., Vol. 47, No. 6, pp. 823-833.

[28] Park, C.-Y., Kim, S.-O. and Kim, B.-J., 2010, “The characteristic of selective attachment and bioleaching for pyrite using indigenous acidophilic bacteria at 42°C,” Econ. Environ. Geol., Vol. 43, No. 2, pp. 109-121.

[29] Park, J.S., 2016, A Novel Process for Manufacturing the Electrolyte for Electrowinning EMM (Eletrolytic Manganese Metal) from El Boleo Mine Waste Water, MSc Thesis, Chonnam National University, Korea, p. 60.

[30] Qin, W., Yang, C., Lai, S., Wang, J., Liu, K. and Zhang, B., 2013, “Bioleaching of chalcopyrite by moderately thermophilic microorganisms,” Bioresour. Technol., Vol. 129, pp. 200-208.

[31] Rohwerder, T., Gehrke, T., Kinzler, K. and Sand, W., 2003, “Bioleaching review part A: Progress in bioleaching: fundamentals and mechanisms of bacterial metal sulfide oxidation,” Appl. Microbiol. Biotechnol., Vol. 63, pp. 239-248.

[32] Salas, R.E.D.R., 2011, Metallogenesis for the Boleo and Cananea Copper Mining Districts: A Contribution to the Understanding of Copper Ore Deposits in Northwestern Mexico, Ph.D. Thesis, The University of Arizona, USA, p. 226.

[33] Sandström, A., Shchukarev, A. and Paul, J., 2005, “XPS characterisation of chalcopyrite chemically and bio-leached at high and low redox potential,” Miner. Eng., Vol. 18, No. 5, pp. 505-515.

[34] Sun, W.Y., Su, S.J., Wang, Q.Y. and Ding, S.L., 2013, “Lab-scale circulation process of electrolytic manganese production with low-grade pyrolusite leaching by SO₂,” Hydrometallurgy, Vol. 133, pp. 118-125.

[35] Vavra, J.P. and Frederick, L.R., 1952, “The effect of sulfur oxidation on the availability of manganese,” Soil Science Society of America Journal, Vol. 16, No. 2, pp. 141-144.

[36] Zhang, W. and Cheng, C.Y., 2007, “Manganese metallurgy review. Part I: Leaching of ores/secondary materials and recovery of electrolytic/chemical manganese dioxide,” Hydrometallurgy, Vol. 89, No. 3, pp. 137-159.

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