Study on Geochemical Characteristics of Paenibacillus hunanensis Grown Under Red,  Green,  and Blue Lights

Hui-Yeon Kim; Ju-Yeong Park; Ji-In Lee; Chanwoo Im; Eui-Jeong Hwang; Yejin Choi; Jong-Un Lee

doi:10.32390/ksmer.2024.61.2.136

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2024 Vol.61, Issue 2 Preview Page Next Page

Technical Report

Study on Geochemical Characteristics of Paenibacillus hunanensis Grown Under Red, Green, and Blue Lights 적색, 녹색, 청색광에서 생장한 Paenibacillus hunanensis의 지구화학적 특성 연구

30 April 2024. pp. 136-142

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Abstract

Bacteria that had evolved over multiple generations under different visible light wavelengths were evaluated for their growth characteristics, resistance to various pH levels, and copper toxicity. The microorganism was isolated from soil at Chonnam National University and identified as Paenibacillus hunanensis with a similarity of 99.2%. Microbial culturing over 31 generations was performed under red, green, and blue light wavelengths, and the biomass of the bacteria grown in each generation was measured. Additionally, the biomass of bacteria grown at pH 4, 7, and 9 and copper concentrations of 10, 100, and 1000 ppb were determined using bacteria from the 31st generation. The results showed that the bacteria grown under red light exhibited higher activity over time. Bacteria grown under red light showed higher biomass at pH 4 and 7. Owing to the differences in resistance to copper, bacteria grown under blue light and in the absence of light exhibited lower biomass.

Keywords

light wavelength

evolution

bacteria

activity

resistance

이 연구에서는 서로 다른 가시광선 파장 조건하에서 세대를 거듭하여 진화한 박테리아의 생장 특성과 다양한 pH 및 구리 독성에 대한 내성을 평가하였다. 전남대학교 내 토양으로부터 분리한 미생물을 실험 대상으로 하였으며 16s rRNA 결과 Paenibacillus hunanensis와 99.2%의 유사도를 보였다. 분리된 토양 토착균을 이용하여 적색, 녹색, 청색광의 파장에서 31세대에 걸쳐 미생물의 계대배양을 수행하였으며 세대별로 생장한 박테리아의 생물량을 측정하였다. 또한 31세대 후의 박테리아를 이용하여 pH 4, 7, 9 및 구리 함량 10, 100, 1000 ppb 조건에서 생장한 생물량을 확인하였다. 실험 결과, 시간이 경과하며 적색광 조건에서 생장한 박테리아가 높은 활성도를 보였으며, 적색광 조건에서 생장한 박테리아는 pH 4와 7에서 높은 생물량을 나타냈다. 구리에 대한 내성 차이를 관찰한 결과, 청색광 조건 및 빛이 없는 조건에서 생장한 박테리아가 낮은 생장량을 보였다.

키워드

빛 파장

진화

박테리아

활성도

내성

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Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 61
No :2
Pages :136-142
Received Date : 2024-02-08
Revised Date : 2024-03-26
Accepted Date : 2024-03-27
DOI :https://doi.org/10.32390/ksmer.2024.61.2.136

Journal of the Korean Society of Mineral and Energy Resources Engineers ISSN:2288-0291(Print) 2288-2790(Online) 한국자원공학회지

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