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2013 Vol.50, Issue 5 Preview Page

Research Paper

October 2013. pp. 651-659
Abstract
This study demonstrates the potential of Ourococcus multisporus YSW008 for removal of nitrogen and phosphorus from different types of wastewaters(municipal wastewater - influent, secondary and tertiary effluents, and piggery wastewater effluent) with simultaneous production of algal biofuel feedstock. Municipal wastewater supported a higher growth(0.2 to 0.22 g/L) compared to the piggery wastewater after 10 days of cultivation. Removal efficiency of nitrogen and phosphorus in municipal wastewater was 94 and 95%, respectively. The microalgal carbohydrate and fatty acid contents cultivated in municipal wastewater were 31 and 9% higher compared to the piggery wastewater. The content of oleic acid was 2.5 times higher in the municipal wastewater than in the piggery wastewater. The results showed that O. multisporus has a higher potential of nitrogen and phosphorus removal from municipal wastewater compared to piggery wastewater, with simultaneous production of algal biomass for biofuel feedstock.
Ourococcus multisporus YSW008(O. multisporus)을 이용한 폐수 내 질소와 인 제거 효율과 생산된 바이오매스의 에너지 전환가능성을 검토하였다. 하수에서 10일 동안 배양 시 바이오매스 생산량은 0.2-0.22 g/L로 양돈폐수에 비해 생산량이 높았으며, 질소, 인 제거효율은 각각 94와 95% 이상으로 나타났다. 바이오매스 내 탄수화물 및 지방산 함량은 하수에서 배양 시 양돈폐수 배출수 적용 시 보다 각각 최대 31 및 9% 증가되었다. 또한, 지방산 성분 중 고품질 바이오디젤 생산에 적합한 올레산(C18:1n9c) 함량이 하수에서 배양 시 양돈폐수 배출수 보다 약 2.5배 이상 증가되었다. O. multisporus 종은 하수에서 배양 시 고도폐수처리 및 고품질 바이오연료 전환에 적합하다고 판단된다.
References
  1. Abou-Shanab, Reda, A.I., Raghavulu, S.V., Hassanin, Nagah, M.A., Kim, S., Kim, Y.J., Oh, S.U., Oh, Y.-K. and Jeon, B.-Y., 2012, “Manipulating nutrient composition of microalgal growth media to improve biomass yield and lipid content of Micractinium pusillum,” African J. of Biotechnology, Vol. 11, pp. 16270-16276.
  2. APHA, 1998, Standard methods for the examination of water and wastewater, American Public Health Association Baltimore, MD, USA.
  3. Behzadi, S. and Farid, M.M., 2007, “Review: examining the use of different feedstock for the production of biodiesel,” Asia-Pacific J. of chemical Engineering, Vol. 2, pp. 480-486.
  4. Bischoff, H.W. and Bold, H.C., 1963, “Phycological Studies IV. Some soil algae from enchanted rock and related algal species,” University of Texas Publication Vol. 6318, pp. 1-95.
  5. Brennan, L. and Owende, P., 2010, “Biofuels from microalgae – a review of technologies for production, processing, and extractions of biofuels and co-products,” Renewable and Sustainable Energy Review, Vol. 14, pp. 557-577.
  6. Brown, M.R., McCausland, M.A. and Kowalski, K., 1998, “The nutritional value of four Australian microalgal strains fed to Pacific oyster Crassostrea gigas spat,” Aqua-culture, Vol. 165, pp. 281-293.
  7. Chen, M., Tang, H., Ma, H., Holland, T.C., Ng, K.Y.S. and Salley, S.O., 2011, “Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta,” Bioresource Technology, Vol. 102, pp. 1649-1655.
  8. Chisti, Y., 2007, “Biodiesel from microalgae,” Biotechnology Advances, Vol. 25, pp. 294-306.
  9. Cho, S., Luong, T.T., Lee, D., Oh, U.–K. and Lee, T., 2011, “Reuse of effluent water from a municipal wastewater treatment plant in microalgae cultivation for biofuel production,” Bioresource Technology, Vol. 102, pp. 8639-8645.
  10. Choi, J.-A., Hwang, J.-H., Dempsey, B.A., Abou-Shanab, Reda, A.I., Min, B., Song, H., Lee, D.S., Kim, J.R., Cho, Y., Hong, S. and Jeon, B.-H., 2011, “Enhancement of fermentative bioenergy(ethanol/hydrogen) production using ultrasonication of Scenedesmus obliquus YSW15 cultivated in swine wastewater effluent,” Energy Environmental Science, Vol. 4, pp. 3513-3520.
  11. Christensen, K.K., 1997, “Differences in iron, manganese, and phosphorus binding in freshwater sediment vegetated with Littorella uniflora and benthic microalgae,” Water, Air, & Soil Pollution, Vol. 99, pp. 265-273.
  12. Chung, J.H., Kwon, G.-S. and Jang, H.-S., 2008, “Development of transportation bio-energy and its future,” Korean J. of Microbiology Biotechnology, Vol. 36, pp. 1-5.
  13. de Morais, M.G. and Costa, J.A., 2007, “Biofixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor,” J. of Biotechnology, Vol. 129, No. 3, pp. 439-445.
  14. Dubios, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F., 1956, “Colormetric method for determination of sugars and related substances,” J. of Analytical Chemistry, Vol. 28, pp. 350-356.
  15. Ho, S.H., Chen, C.Y. and Chang, J.S., 2012, “Effect of light intensity and nitrogen starvationon CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N,” Bioresource Technology, Vol. 113, pp. 244–252.
  16. Hu, Q., 2004, “Environmental effects on cell composition, in: Richmond, A.(Ed.), Handbook of Microalgal Culture: Biotechnology and Applied Phycology,” Blackwell Publishing, Oxford, pp. 83-93.
  17. Illman, A.M., Scragg, A.H. and Shales, S.W., 2000, “Increase in Chlorella strains calorific values when grown in low nitrogen medium,” Enzyme and Microbial Technology, Vol. 27, pp. 631-635.
  18. Ji, M.-K., Abou-Shanab, Reda, A.I., Kim, S.-H., Salama, El-Sayed, Lee, S.-H., Kabra, Akhil, N. and Jeon, B.-H., 2013a, “Cultivation of microalgae species in tertiary municipal wastewater supplemented with CO2 for nutrient removal and biomass production,” Ecological Engineering, accepted, DOI 10.1016/j.ecoleng. 2013.06.020.
  19. Ji, M.-K., Kim, H.-C., Sapireddy, V.R., Yun, H.-S., Abou-Shanab, Reda, A.I., Choi, J., Lee, W., Timmes, T.C., Inamuddin. and Jeon, B.-H., 2013b, “Simultaneous nutrient removal and lipid production from pretreated piggery wasteawater by Chlorella vulgaris YSW-04,” Applied Microbiology Biotechnology, Vol. 97, No. 6, pp. 2701-2710.
  20. Ji, M.-K., Abou-Shanab, Reda, A.I., Hwang, J.-H., Timmes, Thomas, C., Kim, H.-C., Oh, Y.-K. and Jeon, B.-H., 2013c, “Removal of nitrogen and phosphorus from piggery wastewater effluent using the green microalga Scenedesmus Obliquus,” J. of Environmental Engineering, Vol. 139, No. 9, pp. 1198-1205.
  21. Jiang, L., Luo, S., Fan, X., Yang, Z. and Guo, R., 2011, “Biomass and lipid production of marine microalgae using municipal wastewater and high concentration of CO2,” Applied Energy, Vol. 88, pp. 3336-3341.
  22. Khan, M. and Yoshida, N., 2008, “Effect of L-glutamic acid on the growth and ammonium removal from ammonium solution and natural wastewater by chlorella vulgaris NTM06,” Bioresource Technology, Vol. 999, pp. 575-582.
  23. Knothe, G., 2008, “Designer biodiesel: optimizing fatty ester composition to improve fuel properties,” Energy Fuels, Vol. 22, pp. 1358-1364.
  24. Lamelas, C. and Slaveykova, V.I., 2007, “Comparison of Cd(Ⅱ), Cu(Ⅱ), and Pb(Ⅱ) biouptake by green algae in the presence of humic acid,” Environmental Science Technology, Vol. 41, No.11, pp. 4172-4178.
  25. Lee, K. and Lee, C.-G., 2001, “Effect of light/dark cycles on wastewater treatments by microalgae,” Biotechnology and Bioprocess Engineering, Vol. 6, No. 3, pp. 194-199.
  26. Lepage, G. and Roy, C.C., 1984, “Improved recovery of fatty acid through direct transesterification without prior extraction or purification,” J. of Lipid Research, Vol. 25, pp. 1391-1396.
  27. Li, X., Hu, H.-Y. and Yang, J., 2010, “Lipid accumulation and nutrient removal properties of a newly isolated freshwater microalga, Scenedesmus sp. LX1, growing in secondary effluent,” New Biotechnology, Vol. 27, No.1, pp. 59-63.
  28. Luque, R., Lovett, J.C., Datta, B., Clancy, J., Campelo, J. M. and Romero, A.A., 2010, “Biodiesel as feasible petrol fuel replacement: a multidisciplinary overview,” Energy Environmental Science, Vol. 3, pp. 1706-1721.
  29. Matusiak, K., Pryztocka-Jusiak, M., Leszczynska-Gerula, K. and Horoch, M., 1976, “Studies on the purification of wastewater from the nitrogen fertilizer industry by intensive algal cultures. II: Removal of nitrogen from wastewater,” Acta Microbiologica Polonica, Vol. 25, pp. 361-374.
  30. Msanne, J., Xu, D., Konda, A.R., Casas-Mollano, J.A., Awada, T., Cahoon, E.B. and Cerutti, H., 2012, “Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169,” Phytochemistry, Vol. 75, pp. 50-59.
  31. Na, J.-G., Lee, H.S., Oh, Y.-K., Park, J.-Y., Ko, C.H., Lee, S.-H., Yi, K.B., Chung, S.H. and Jean, S.G., 2011, “Rapid estimation of triacylglycerol content of Chlorella sp. by thermogravimetric analysis,” Biotechnology Letters, Vol. 33, pp. 957-960.
  32. Oswald, W.J., 2003, “My sixty years in applied algology,” J. of Applied Phycology, Vol. 15, pp. 99-106.
  33. Park, J., Jin, H.F., Lim, B.R., Park, K.Y. and Lee, K., 2010, “Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp.,” Bioresource Technology, Vol. 101, pp. 8649-8657.
  34. Rashid, U., Anwar, F., Moser, B.R. and Knothe, G., 2008, “Moringa oleifera oil: a possible source of biodiesel,” Bioresource Technology, Vol. 99, pp. 8175-8179.
  35. Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G. and Tredici, M.R., 2009, “Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor,” Biotechnology Bioengineering, Vol. 102, pp. 100-112.
  36. Rosegrant, M.W., Msangi, S., Sulser, T. and Valmonte-Santos, R., 2006, “Biofuels and the Gloval Food Balance,” International Food Policy Research Institute, Washington DC, USA.
  37. Sun, Y. and Cheng, J.Y., 2002, “Hydrolysis of lignocellulosic materials for ethanol production: a review,” Bioresource Technology, Vol. 83, pp. 1-11.
  38. Singh, S.P. and Singh, D., 2010, “Biodiesel production through the use of different sources and characterization of oils and their esters as the substitute of diesel: A review,” Renewable and Sustainable Energy Review, Vol. 14, pp. 200-216.
  39. Somerville, C., 2006, “The billion-ton biofuels vision,” Science, Vol. 312, p. 1277.
  40. Voltolina, D., Gómez-Villa. and Correa, G., 2005, “Nitrogen removal and recycling by Scenedesmus obliquus in semicontinous cultures using artificial wastewater and a simulated light and temperature cycle,” Bioresource Technology, Vol. 96, No. 3, pp. 359-362.
  41. Wang, J.V., Miller, T.W., Hobbs, S., Hook, P., Crowe, B. and Huesemann, M., 2012, “Effects of light and temperature on fatty acid production in Nannochloropsis Salina,” Energies, Vol. 5, pp. 731-740.
  42. Wilhelm, C. and Jakob, T., 2011, “From photons to biomass and biofuels: evaluation of different strategies for the improvement of algal biotechnology based on comparative energy balances,” Applied Microbiology and Biotechnology, Vol. 92, No. 5, pp. 909-919.
  43. Williams, P.J. le B. and Laurens, L.M.L., 2010, “Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics,” Energy Environmental Science, Vol. 3, pp. 554-590.
  44. Yeesang, C. and Cheirsilp, B., 2011, “Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated freshwater sources in Thailand,” Bioresource Technology, Vol. 102, pp. 3034-3040.
Information
  • Publisher :The Korean Society of Mineral and Energy Resources Engineers
  • Publisher(Ko) :한국자원공학회
  • Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
  • Journal Title(Ko) :한국자원공학회지
  • Volume : 50
  • No :5
  • Pages :651-659