The Characteristic of Bioleaching for Chalcopyrite Concentrate Using Indigenous Acidophilic Bacteria - Column Leaching at Room Temperature -

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2010 Vol.47, Issue 5 Preview Page Next Page

The Characteristic of Bioleaching for Chalcopyrite Concentrate Using Indigenous Acidophilic Bacteria - Column Leaching at Room Temperature - 토착호산성 박테리아를 이용한 황동석 정광에 대한 생물학적 용출 특성 - 상온에서의 칼럼 용출 -: 한오형, 박천영, 조강희
Oh-Hyung Han, Cheon-Young Park and Kang-Hee Cho

31 October 2010. pp. 678-689

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Abstract

This study was to investigate the bioleaching for chalcopyrite using subcultured indigenous acidophilic bacteria without addition of sulfuric acid at room temperature. Even through these condition, the possibility of bioleaching was identified to leach the usable metal ions from chalcopyrite concentrate. The indigenous bacteria were collected from the acid mine drainage, mine waste soil, coal waste and hot spring in the Korea and Japan, and cultured in the growth-medium at 32℃. On the indigenous bacteria growing in the growth-medium at 32℃, the pH value was gradually decreased over time and the Eh value increased with time. After 28 days inoculation with chalcopyrite column without sulfuric acid at room temperature, the content of Fe, Zn, Cu and As enriched 184, 6, 3.3 and 2.7 times more in the bacterial sample than the control sample. Accordingly, it is respected that the heap and dump leaching will be possible to leach the usable metal ions from the experiment no addition sulfur acid at room temperature.

Keywords

Indigenous acidophilic bacteria

Column bioleaching

Chalcopyrite

Room temperature

계대배양 된 토착박테리아들을 황동석이 충진 된 칼럼에 접종하고 상온에서 그리고 황산을 첨가하지 않고 용출실험을 실시하였다. 비록 이런 조건에도 불구하고, 미생물에 의하여 황동석 정광으로부터 유용금속 이온이 용출될 수 있는지를 확인하고자 하였다. 한국과 일본지역에서 광산산성배수, 광산폐석토양, 폐석탄 및 온천수에 서식하는 토착박테리아들을 성장-배양액에서 32℃로 배양하였다. 성장-배양액에서 토착박테리아가 성장함에 따라 pH가 감소하고 Eh는 증가하였다. 토착박테리아를 황동석 칼럼에 28일 동안 반응시킨 결과 비교시료에서 보다 박테리아 시료에서 Fe 이온은 184 배 이상으로, Zn은 6배 이상으로, Cu는 3.3 배 이상으로 그리고 As는 2.7배 이상으로 용출되었다. 따라서, 상온에서 그리고 황산을 첨가하지 않은 실험 조건에서 heap leaching이나 dump leaching으로부터 유용금속을 용출시킬 수 있을 것으로 기대된다.

키워드

토착호산성박테리아

칼럼미생물용출

황동석

실험실온도

References

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기상청, 2010.04.01, www.kma.go.kr
박천영, 정경훈, 김계민, 홍영의, 조강희, 2009, “화순 광산배수에 서식하는 토착 호산성 박테리아를 이용한 황철석의 용출 특성,” 한국지구시스템공학회지, 제46권 5호, pp. 521-535.
Bennett, J.C. and Tributsch, H., 1978, “Bacterial leaching patterns on pyrite crystal surfaces,” Journal of Bacteriology, Vol. 134, pp. 310-317.
Berry, V.K. and Murr, L.E., 1978, “Direct observations of bacteria and quantitative studies of their catalytic role in the leaching of low-grade, copper-bearing waste,” L.E. Murr, A.E. Torma and A. Brierley, (eds)., Merallurgical Applications of Bacterial Leaching and Related Microbiological Phenomena, Academic press, New York, pp. 103-136.
Brierley, C.L., 1978, “Bacterial leaching,” Critical Reviews in Microbiology, Vol. 6, pp. 207-262.
Brierley, C.L., 1982, “Microbiological mining,” Scientific American, Vol. 247, pp. 42-51.
Brierley, J.A., 2003, “Response of microbial systems to thermal stress in heap-biooxidation pretreatment of refractory gold ores,” Hydrometallurgy, Vol. 71, pp. 13-19.
Brierley, J.A. and Brierley, C.L., 1978, “Microbiol leaching of copper at ambient and elevated temperatures,” In Murr, L.E., Torma, A.E. and Brierley, J.A. (eds.), Metallurgical Applications of Bacterial Leaching and Related Microbiological Phenomena, Academic Press, New York, pp. 477-490.
Brierley, J.A. and Brierley, C.L., 2001, “Present and future commercial applications of biohydrometallurgy,” Hydrometallurgy, Vol. 59, pp. 233-239.
Bruynesteyn, A. and Duncan, D.W., 1971, “Microbiological leaching of sulfide minerals,” Canadian Metallurgical Quarterly, Vol. 10, pp. 57-63.
Devasia, P., Natarajan, K.A., Sathyanarayana, D.N. and Rao, G.R., 1993, “Surface chemistry of Thiobacillus ferrooxidans relevant to adhesion on mineral surface,” Applied and Environmental Microbiology, Vol. 59, pp. 4051-4055.
Edwards, K.J., Hu, B., Hamers, R.J. and Banfield, J.F., 2001, “A new look at microbial leaching patterns on
sulfide minerals,” FEMS Microbiology Ecology, Vol. 34, pp. 197-206.
Faure, G., 1991, “Principles and application of inorganic geochemistry,” Macmillan Publishing Company, 626p.
Groudev, S.N. and Groudeva, V.I., 1993, “Microbial communities in four industrial copper dump leaching operations in Bulgaria,” FEMS Microbiology Reviews, Vol. 11, pp. 261-268.
Healy, T.W. and Moignard, M.S., 1976, “A review of electrokinetic studies of metal sulphides,” In: Fuerstenau, M.C.(eds), Flotation American Institute of Mining, Metallurgical, and Petroleum Engineers, Inc. pp. 275-297.
Hosseini, T. R., Kolahdoozan, M., Tabatabaei, Y. S. M., Oliazadeh, M., Noaparast, M., Eslami, A., Manafi, Z. and Alfantazi, A., 2005, “Bioflotation of Sarcheshmeh copper using Thiobacillus ferrooxidans bacteria,” Minerals Engineering, Vol. 18, pp. 371-374.
Langmuir, D., 1997, “Aqueous environmental geochemistry,” Prentice Hall, 600p.
Leduc, L.G. and Ferroni, G.D., 1994, “The chemolithotrophic bacterium Thiobacillus ferrooxidans,” FEMS Microbiology Reviews, Vol. 14, pp. 103-120.
Lizama, H.M. and Suzuki, I., 1988, “Bacterial leaching of a sulphide ore by Thiobacillus ferrooxidans: I. Shake flask studies,” Biotechnology and Bioengineering, Vol. 32, pp. 110-116.
Lizama, H.M. and Suzuki, I., 1989, “Bacterial leaching of a sulfide ore by Thiobacillus ferrooxidans and Thiobacillus thiooxidans. Part 2. Column leaching studies,” Hydrometallurgy, Vol. 22, pp. 301-310.
Lizama, H.M. and Suzuki, I., 1990, “Interaction of chalcopyrite and sphalerite with pyrite during leaching by Thiobacillus ferrooxidans and Thiobacillus thiooxidans,” Canadian Journal Micobiology, Vol. 37, pp. 304-311.
Marsden, J. and House, I., 1992, “The chemistry of gold extraction,” Ellis Horwood, 597p.
Mozes, N. and Rouxhet, P.G., 1987, “Methods for measuring hydrophobicity of microorganisms,” Journal of Micro-Biological Methods, Vol. 6, pp. 99-112.
Murr, L.E. and Berry, V.K., 1976, “Direct observations of selective attachment of bacteria on low-grade sulfide ores and other mineral surfaces,” Hydrometallurgy, Vol. 2, pp. 11-24.
Ohmura, N., Kitamura, K. and Saiki, H., 1993, “Mechanism of microbial flotation using Thiobacillus ferrooxidans for pyrite supperession,” Biotechnology and Bioengineering, Vol. 41, pp. 671-676.
Rosenberg, M. and Doyle, R.J., 1990, “Microbial cell surface hydrophobicity: history, measurement, and significance,” In; Doyle, R.J. and Rosenberg, M., (eds), Microbial cell surface hydrophobicity. American Society for Microbiology, Washington, D.C. pp. 1-37.
Rouf, M.A. and Stokes, J.L., 1964, “Morphology nutrition and physiology of Sphaerotilus discophorus,” Archives of Microbiology, Vol. 49, pp. 132-149.
Sabatini, D.D., Bensch, K. and Barrnett, R.J., 1963, “Cytochemistry and electron microscopy, the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation,” The Journal of Cell Biology, Vol. 17, pp. 19-58.
Schippers, A., 2007, “Microorganisms involved in bioleaching and nucleic acid-based molecular methods for their identification and quantification,” In Donati, E.R. and Sand, W. (eds.), Microbial processing of metal sulfides, Springer, pp. 3-33.
Schippers, A., Hallmann, R., Wentzien, S. and Sand, W., 1995, “Microbial diversity in uranium mine waste heap,” Applied and Environmental Microbiology, Vol. 61, pp. 2930-2935.
Shrihari, S., Kumar, R., Ghandi, K.S. and Natarajan, K.A., 1991, “Role of cell attachment in leaching of chalcopyrite mineral by Thiobacillus ferrooxidans,” Applied and Microbiology Biotechnology, Vol. 36, pp. 278-282.
Silverman, M.P., 1967, “Mechanism of bacteria pyrite oxidation,” Journal of Bacteriology, Vol. 94, pp. 1046-1051.
Silverman, M.P. and Ehrlich, H.L., 1964, “Microbial formation and degradation of minerals,” Advan. Appl. Microbiol., Vol. 6, pp. 153-206.
Torma, A.E. Ashman, P.R., Olson, T.M. and Bosecker, K., 1979, “Microbiological leaching of a chalcopyrite concentrate and recovery of copper by solvent extraction and electrowinning,” Metall, Vol. 33, pp. 479-484.
Van Loosdrecht, M.C.M., Lyklema, J., Norde, W., Schraa, G. and Zehnder, A.J.B., 1987, “The role of bacterial cell wall hydrophobicity in adhesion,” Applied and Environmental Microbiology, Vol. 53, pp. 1893-1897.
Wakao, N., Mishina, M., Sakurai, Y. and Shiota, H., 1982, “Bacterial pyrite oxidation. 1. The effect of pure and mixed cultures of Thiobacillus ferrooxidans and Thiobacillus thiooxidans on release of iron,” J Gen Appl Microbiol., Vol. 28, pp. 331-343.

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society for Geosystem Engineering
Journal Title(Ko) :한국지구시스템공학회지
Volume : 47
No :5
Pages :678-689

[1] 고명수, 박현성, 이종운, 2009, “황산화균 Acidthiobacillus thiooxidans를 이용한 폐금은광산 광미에서의 중금속용 출,” 한국지구시스템공학회지, 제46권 2호, pp. 239-251.

[2] 기상청, 2010.04.01, www.kma.go.kr

[3] 박천영, 정경훈, 김계민, 홍영의, 조강희, 2009, “화순 광산배수에 서식하는 토착 호산성 박테리아를 이용한 황철석의 용출 특성,” 한국지구시스템공학회지, 제46권 5호, pp. 521-535.

[4] Bennett, J.C. and Tributsch, H., 1978, “Bacterial leaching patterns on pyrite crystal surfaces,” Journal of Bacteriology, Vol. 134, pp. 310-317.

[5] Berry, V.K. and Murr, L.E., 1978, “Direct observations of bacteria and quantitative studies of their catalytic role in the leaching of low-grade, copper-bearing waste,” L.E. Murr, A.E. Torma and A. Brierley, (eds)., Merallurgical Applications of Bacterial Leaching and Related Microbiological Phenomena, Academic press, New York, pp. 103-136.

[6] Brierley, C.L., 1978, “Bacterial leaching,” Critical Reviews in Microbiology, Vol. 6, pp. 207-262.

[7] Brierley, C.L., 1982, “Microbiological mining,” Scientific American, Vol. 247, pp. 42-51.

[8] Brierley, J.A., 2003, “Response of microbial systems to thermal stress in heap-biooxidation pretreatment of refractory gold ores,” Hydrometallurgy, Vol. 71, pp. 13-19.

[9] Brierley, J.A. and Brierley, C.L., 1978, “Microbiol leaching of copper at ambient and elevated temperatures,” In Murr, L.E., Torma, A.E. and Brierley, J.A. (eds.), Metallurgical Applications of Bacterial Leaching and Related Microbiological Phenomena, Academic Press, New York, pp. 477-490.

[10] Brierley, J.A. and Brierley, C.L., 2001, “Present and future commercial applications of biohydrometallurgy,” Hydrometallurgy, Vol. 59, pp. 233-239.

[11] Bruynesteyn, A. and Duncan, D.W., 1971, “Microbiological leaching of sulfide minerals,” Canadian Metallurgical Quarterly, Vol. 10, pp. 57-63.

[12] Devasia, P., Natarajan, K.A., Sathyanarayana, D.N. and Rao, G.R., 1993, “Surface chemistry of Thiobacillus ferrooxidans relevant to adhesion on mineral surface,” Applied and Environmental Microbiology, Vol. 59, pp. 4051-4055.

[13] Edwards, K.J., Hu, B., Hamers, R.J. and Banfield, J.F., 2001, “A new look at microbial leaching patterns on

[14] sulfide minerals,” FEMS Microbiology Ecology, Vol. 34, pp. 197-206.

[15] Faure, G., 1991, “Principles and application of inorganic geochemistry,” Macmillan Publishing Company, 626p.

[16] Groudev, S.N. and Groudeva, V.I., 1993, “Microbial communities in four industrial copper dump leaching operations in Bulgaria,” FEMS Microbiology Reviews, Vol. 11, pp. 261-268.

[17] Healy, T.W. and Moignard, M.S., 1976, “A review of electrokinetic studies of metal sulphides,” In: Fuerstenau, M.C.(eds), Flotation American Institute of Mining, Metallurgical, and Petroleum Engineers, Inc. pp. 275-297.

[18] Hosseini, T. R., Kolahdoozan, M., Tabatabaei, Y. S. M., Oliazadeh, M., Noaparast, M., Eslami, A., Manafi, Z. and Alfantazi, A., 2005, “Bioflotation of Sarcheshmeh copper using Thiobacillus ferrooxidans bacteria,” Minerals Engineering, Vol. 18, pp. 371-374.

[19] Langmuir, D., 1997, “Aqueous environmental geochemistry,” Prentice Hall, 600p.

[20] Leduc, L.G. and Ferroni, G.D., 1994, “The chemolithotrophic bacterium Thiobacillus ferrooxidans,” FEMS Microbiology Reviews, Vol. 14, pp. 103-120.

[21] Lizama, H.M. and Suzuki, I., 1988, “Bacterial leaching of a sulphide ore by Thiobacillus ferrooxidans: I. Shake flask studies,” Biotechnology and Bioengineering, Vol. 32, pp. 110-116.

[22] Lizama, H.M. and Suzuki, I., 1989, “Bacterial leaching of a sulfide ore by Thiobacillus ferrooxidans and Thiobacillus thiooxidans. Part 2. Column leaching studies,” Hydrometallurgy, Vol. 22, pp. 301-310.

[23] Lizama, H.M. and Suzuki, I., 1990, “Interaction of chalcopyrite and sphalerite with pyrite during leaching by Thiobacillus ferrooxidans and Thiobacillus thiooxidans,” Canadian Journal Micobiology, Vol. 37, pp. 304-311.

[24] Marsden, J. and House, I., 1992, “The chemistry of gold extraction,” Ellis Horwood, 597p.

[25] Mozes, N. and Rouxhet, P.G., 1987, “Methods for measuring hydrophobicity of microorganisms,” Journal of Micro-Biological Methods, Vol. 6, pp. 99-112.

[26] Murr, L.E. and Berry, V.K., 1976, “Direct observations of selective attachment of bacteria on low-grade sulfide ores and other mineral surfaces,” Hydrometallurgy, Vol. 2, pp. 11-24.

[27] Ohmura, N., Kitamura, K. and Saiki, H., 1993, “Mechanism of microbial flotation using Thiobacillus ferrooxidans for pyrite supperession,” Biotechnology and Bioengineering, Vol. 41, pp. 671-676.

[28] Rosenberg, M. and Doyle, R.J., 1990, “Microbial cell surface hydrophobicity: history, measurement, and significance,” In; Doyle, R.J. and Rosenberg, M., (eds), Microbial cell surface hydrophobicity. American Society for Microbiology, Washington, D.C. pp. 1-37.

[29] Rouf, M.A. and Stokes, J.L., 1964, “Morphology nutrition and physiology of Sphaerotilus discophorus,” Archives of Microbiology, Vol. 49, pp. 132-149.

[30] Sabatini, D.D., Bensch, K. and Barrnett, R.J., 1963, “Cytochemistry and electron microscopy, the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation,” The Journal of Cell Biology, Vol. 17, pp. 19-58.

[31] Schippers, A., 2007, “Microorganisms involved in bioleaching and nucleic acid-based molecular methods for their identification and quantification,” In Donati, E.R. and Sand, W. (eds.), Microbial processing of metal sulfides, Springer, pp. 3-33.

[32] Schippers, A., Hallmann, R., Wentzien, S. and Sand, W., 1995, “Microbial diversity in uranium mine waste heap,” Applied and Environmental Microbiology, Vol. 61, pp. 2930-2935.

[33] Shrihari, S., Kumar, R., Ghandi, K.S. and Natarajan, K.A., 1991, “Role of cell attachment in leaching of chalcopyrite mineral by Thiobacillus ferrooxidans,” Applied and Microbiology Biotechnology, Vol. 36, pp. 278-282.

[34] Silverman, M.P., 1967, “Mechanism of bacteria pyrite oxidation,” Journal of Bacteriology, Vol. 94, pp. 1046-1051.

[35] Silverman, M.P. and Ehrlich, H.L., 1964, “Microbial formation and degradation of minerals,” Advan. Appl. Microbiol., Vol. 6, pp. 153-206.

[36] Torma, A.E. Ashman, P.R., Olson, T.M. and Bosecker, K., 1979, “Microbiological leaching of a chalcopyrite concentrate and recovery of copper by solvent extraction and electrowinning,” Metall, Vol. 33, pp. 479-484.

[37] Van Loosdrecht, M.C.M., Lyklema, J., Norde, W., Schraa, G. and Zehnder, A.J.B., 1987, “The role of bacterial cell wall hydrophobicity in adhesion,” Applied and Environmental Microbiology, Vol. 53, pp. 1893-1897.

[38] Wakao, N., Mishina, M., Sakurai, Y. and Shiota, H., 1982, “Bacterial pyrite oxidation. 1. The effect of pure and mixed cultures of Thiobacillus ferrooxidans and Thiobacillus thiooxidans on release of iron,” J Gen Appl Microbiol., Vol. 28, pp. 331-343.

Journal of the Korean Society of Mineral and Energy Resources Engineers ISSN:2288-0291(Print) 2288-2790(Online) 한국자원공학회지

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