Study on Surface Tension Decrease by Indigenous Bacteria isolated from Petroleum
Reservoir of Myanmar for Microbial Enhanced Oil Recovery

doi:10.12972/ksmer.2015.52.1.056

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2015 Vol.52, Issue 1 Preview Page Next Page

Research Paper

Study on Surface Tension Decrease by Indigenous Bacteria isolated from Petroleum Reservoir of Myanmar for Microbial Enhanced Oil Recovery 미생물학적 원유회수증진을 위한 미얀마 저류층에서 분리한토착미생물에 의한 표면장력 감소특성 연구: 조정희 · 이종운 · 김현태
Jeonghee Jo, Jong-Un Lee and Hyun-Tae Kim

28 February 2015. pp. 56-67

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Abstract

For application to MEOR (microbial enhanced oil recovery) technology, the ability of biosurfactants,which were produced by indigenous bacteria isolated from a crude oil reservoir in Myanmar, to decrease surfacetension was investigated. When various carbon sources were supplied to the bacterial isolates, Bacillus spp.,designated as BS1 and BS2, showed high biomass growth and decrease in surface tension under glucose supply.Effect of pH and temperature on variation of surface tension was examined under the conditions of 100°C andpH 2, 4, 7, and 11. As a result, the biosurfactant produced by BS1 exhibited stable variation of surface tension,showing relatively low effect under various conditions. The biosurfactants produced by each bacterial isolate wereseparated and purified, and BS1 and BS2 produced the yield of biosurfactant of 0.51 g/L and 0.26 g/L. CMCvalue of biosurfactants produced by BS1 and BS2 were 2.0 g/L and 0.5 g/L, respectively.

Keywords

Indigenous bacteria

Microbial enhanced oil recovery

Biosurfactant

Surface tension

미얀마 저류층에서 분리한 토착미생물이 생성하는 생물계면활성제의 표면장력 감소 능력을 조사함으로써 미생물학적 원유회수증진 기술에 대한 적용 가능성을 살펴 보았다. 탄소원의 종류에 따른 미생물의 생장과표면장력 변화를 측정한 결과, 포도당을 탄소원으로 공급하였을 떄 Bacillus spp.인 BS1과 BS2가 높은 성장률을보였으며 표면장력을 감소시키는 것으로 관찰되었다. 고온과 pH 요인이 표면장력 변화에 미치는 영향을 평가하기 위하여 배양액에서 미생물을 제거한 후 100°C의 온도 및 다양한 pH 조건에 노출시킨 결과, BS1의 생물계면활성제가 전반적으로 높은 희석비율에도 안정적인 표면장력 변화를 보여 상대적으로 외부요인의 영향을 적게받는 것으로 나타났다. BS1과 BS2가 생성한 생물계면활성제를 추출, 정제한 결과 각각 0.51 g/L, 0.26 g/L이얻어졌고 CMC 값은 각각 2.0 g/L, 0.5 g/L로 나타났다.

키워드

토착미생물

미생물학적 원유회수증진

생물계면활성제

표면장력

References

Armstrong, R.T. and Wildenschild, D., 2012, “Investigating the pore-scale mechanisms of microbial enhanced oil recovery,” Journal of Petroleum Science and Engineering, Vol. 94-95, pp. 155-164.
Banat, I.M., 1995, “Biosurfactant production and possible uses in microbial enhanced oil recovery and oil pollution remediation - a review,” Bioresource Technology, Vol. 51, No. 1, pp. 1-12.
Bass, C. and Lappin-Scott, H., 1997, “The bad guys and the good guys in petroleum microbiology,” Oilfield Review, Spring, Vol. 9, No. 1, pp. 17–25.
Bodour, A.A. and Miller-Maier, R.M., 1998, “Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms,” Journal of Microbiological Methods, Vol. 32, No. 3, pp. 273-280.
Brown, L.R., 2010, “Microbial enhanced oil recovery (MEOR),” Current Opinion in Microbiology, Vol. 13, No. 3, pp. 316-320.
Castorena, C.G., Zapata, P.I., Roldán, C.T., Reyes, A.J., Mayol, C.M., Román, V.S. and Olguín, L.P., 2012, “Evaluation of indigenous anaerobic microorganisms from Mexican carbonate reservoirs with potential MEOR application,” Journal of Petroleum Science and Engineering, Vol. 81, pp. 86-93.
Coates, J.D., Chisholm, J.L., Knapp, R.M., McInerney, M.J., Menzie, D.E. and Bhupathiraju, V.K., 1993, “Microbially enhanced oil recovery field pilot, Payne County, Oklahoma,” Developments in Petroleum Science, Vol. 39, pp. 197-205.
Desai, J.D. and Banat, I.M., 1997, “Microbial production of surfactant and their commercial potential,” Microbiology and Molecular Biology Reviews, Vol. 61, No. 1, pp. 47-64.
Ibrahim, M.L., Ijah, U.J.J., Manga, S.B., Bilbis, L.S. and Umar, S., 2013, “Production and partial characterization of biosurfactant produced by crude oil degrading bacteria,” International Biodeterioration & Biodegradation, Vol. 81, pp. 28-34.
Kim, D.-W., Kim, M.-J. and Kang, S.-M., 1995, “Purification and Properties of Biosurfactant from Pseudomonas aeruginosa KK-7,” Journal of Microbiology and Biotechnology, Vol. 23, No. 3, pp. 337-345.
Lee, K.S., 2010, “Comparison of Reservoir Performances for Chemical Enhanced Oil Recovery,” Journal of The Korean Society for Geosystem Engineering, Vol. 47, No. 3, pp. 291-299.
Makkar, R.S. and Cameotra, S.S., 1999, “Biosurfactant production by microorganisms on unconventional carbon source-a review,” Journal of Surfactant and Detergents, Vol. 2, No. 2, pp. 237-241.
Maure. A., Saldana, A.A. and Juarez, A.R., 2005, “Biotechnology applications to EOR in Talara off-shore oil fields, Northwest Peru,” SPE 94934 presented the 2005 SPE Latin American and Caribbean Petroleum Engineering Conference, Rio de Janeiro, Brazil, June 20-23.
Mulligan, C.N., 2005, “Environmental applications for biosurfactants,” Environmental Pollution, Vol. 133, No. 2, pp. 183-198.
Pornsunthorntawee, O., Arttaweeporn, N., Paisanjit, S., Somboonthanate, P., Abe, M., Rujiravanit, R. and Chavadej, S., 2008, “Isolation and comparison of biosurfactants produced by Bacillus subtilis PT2 and Pseudomonas aeruginosa SP4 for microbial surfactant-enhanced oil recovery,” Biochemical Engineering Journal, Vol. 42, No. 2, pp. 172-179.
Ron, E.Z. and Rosenberg, E., 2001, “Natural roles of biosurfactants,” Environmental Microbiology, Vol. 3, No. 4, pp. 229-236.
Sarafzadeh, P., Hezave, A.Z., Ravanbakhsh, M., Niazi, A. and Ayatollahi, S., 2013, “Enterobacter cloacae as biosurfactant producing bacterium: Differentiating its effects on interfacial tension and wettability alteration mechanisms for oil recovery during MEOR process,” Colloids and Surfaces B: Biointerfaces, Vol. 105, pp. 223-229.
Sen, R., 2008, “Biotechnology in petroleum recovery: The microbial EOR,” Progress in Energy and Combustion Science, Vol. 34, No. 6, pp. 714-724.
Sheppard, J.D. and Mulligan, C.N., 1987, “The production of surfactin by Bacillus subtilis grown on peat hydrolysate,” Applied Microbiology and Biotechnology, Vol. 27, No. 2, pp. 110-116.
Singh, A., Van Hamme, J.D. and Ward, O.P., 2007, “Surfactants in microbiology and biotechnology: Part 2. Application aspects,” Biotechnology Advances, Vol. 25, No. 1, pp. 99-121.
Soudmand-asli, A., Ayatollahi, S.S., Mohabatkar, H., Zareie, M. and Shariatpanahi, S.F., 2007, “The in situ microbial enhanced oil recovery in fractured porous media,” Journal of Petroleum Science and Engineering, Vol. 58, No. 1-2, pp. 161-172.
Tamura, K., Dudley, J., Nei, M. and Kumar, S., 2007, “MEGA 4: Molecular evolutionary genetics analysis (MEGA) software version 4.0.,” Molecular Biology and Evolution, Vol. 24, pp. 1596-1599.
Thompson, J.D., Higgins, D.G. and Gibson, T.J., 1994, “CLUST W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Research, Vol. 22, No. 22, pp. 4673-4680.

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 52
No :1
Pages :56-67
DOI :https://doi.org/10.12972/ksmer.2015.52.1.056

[1] Armstrong, R.T. and Wildenschild, D., 2012, “Investigating the pore-scale mechanisms of microbial enhanced oil recovery,” Journal of Petroleum Science and Engineering, Vol. 94-95, pp. 155-164.

[2] Banat, I.M., 1995, “Biosurfactant production and possible uses in microbial enhanced oil recovery and oil pollution remediation - a review,” Bioresource Technology, Vol. 51, No. 1, pp. 1-12.

[3] Bass, C. and Lappin-Scott, H., 1997, “The bad guys and the good guys in petroleum microbiology,” Oilfield Review, Spring, Vol. 9, No. 1, pp. 17–25.

[4] Bodour, A.A. and Miller-Maier, R.M., 1998, “Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms,” Journal of Microbiological Methods, Vol. 32, No. 3, pp. 273-280.

[5] Brown, L.R., 2010, “Microbial enhanced oil recovery (MEOR),” Current Opinion in Microbiology, Vol. 13, No. 3, pp. 316-320.

[6] Castorena, C.G., Zapata, P.I., Roldán, C.T., Reyes, A.J., Mayol, C.M., Román, V.S. and Olguín, L.P., 2012, “Evaluation of indigenous anaerobic microorganisms from Mexican carbonate reservoirs with potential MEOR application,” Journal of Petroleum Science and Engineering, Vol. 81, pp. 86-93.

[7] Coates, J.D., Chisholm, J.L., Knapp, R.M., McInerney, M.J., Menzie, D.E. and Bhupathiraju, V.K., 1993, “Microbially enhanced oil recovery field pilot, Payne County, Oklahoma,” Developments in Petroleum Science, Vol. 39, pp. 197-205.

[8] Desai, J.D. and Banat, I.M., 1997, “Microbial production of surfactant and their commercial potential,” Microbiology and Molecular Biology Reviews, Vol. 61, No. 1, pp. 47-64.

[9] Ibrahim, M.L., Ijah, U.J.J., Manga, S.B., Bilbis, L.S. and Umar, S., 2013, “Production and partial characterization of biosurfactant produced by crude oil degrading bacteria,” International Biodeterioration & Biodegradation, Vol. 81, pp. 28-34.

[10] Kim, D.-W., Kim, M.-J. and Kang, S.-M., 1995, “Purification and Properties of Biosurfactant from Pseudomonas aeruginosa KK-7,” Journal of Microbiology and Biotechnology, Vol. 23, No. 3, pp. 337-345.

[11] Lee, K.S., 2010, “Comparison of Reservoir Performances for Chemical Enhanced Oil Recovery,” Journal of The Korean Society for Geosystem Engineering, Vol. 47, No. 3, pp. 291-299.

[12] Makkar, R.S. and Cameotra, S.S., 1999, “Biosurfactant production by microorganisms on unconventional carbon source-a review,” Journal of Surfactant and Detergents, Vol. 2, No. 2, pp. 237-241.

[13] Maure. A., Saldana, A.A. and Juarez, A.R., 2005, “Biotechnology applications to EOR in Talara off-shore oil fields, Northwest Peru,” SPE 94934 presented the 2005 SPE Latin American and Caribbean Petroleum Engineering Conference, Rio de Janeiro, Brazil, June 20-23.

[14] Mulligan, C.N., 2005, “Environmental applications for biosurfactants,” Environmental Pollution, Vol. 133, No. 2, pp. 183-198.

[15] Pornsunthorntawee, O., Arttaweeporn, N., Paisanjit, S., Somboonthanate, P., Abe, M., Rujiravanit, R. and Chavadej, S., 2008, “Isolation and comparison of biosurfactants produced by Bacillus subtilis PT2 and Pseudomonas aeruginosa SP4 for microbial surfactant-enhanced oil recovery,” Biochemical Engineering Journal, Vol. 42, No. 2, pp. 172-179.

[16] Ron, E.Z. and Rosenberg, E., 2001, “Natural roles of biosurfactants,” Environmental Microbiology, Vol. 3, No. 4, pp. 229-236.

[17] Sarafzadeh, P., Hezave, A.Z., Ravanbakhsh, M., Niazi, A. and Ayatollahi, S., 2013, “Enterobacter cloacae as biosurfactant producing bacterium: Differentiating its effects on interfacial tension and wettability alteration mechanisms for oil recovery during MEOR process,” Colloids and Surfaces B: Biointerfaces, Vol. 105, pp. 223-229.

[18] Sen, R., 2008, “Biotechnology in petroleum recovery: The microbial EOR,” Progress in Energy and Combustion Science, Vol. 34, No. 6, pp. 714-724.

[19] Sheppard, J.D. and Mulligan, C.N., 1987, “The production of surfactin by Bacillus subtilis grown on peat hydrolysate,” Applied Microbiology and Biotechnology, Vol. 27, No. 2, pp. 110-116.

[20] Singh, A., Van Hamme, J.D. and Ward, O.P., 2007, “Surfactants in microbiology and biotechnology: Part 2. Application aspects,” Biotechnology Advances, Vol. 25, No. 1, pp. 99-121.

[21] Soudmand-asli, A., Ayatollahi, S.S., Mohabatkar, H., Zareie, M. and Shariatpanahi, S.F., 2007, “The in situ microbial enhanced oil recovery in fractured porous media,” Journal of Petroleum Science and Engineering, Vol. 58, No. 1-2, pp. 161-172.

[22] Tamura, K., Dudley, J., Nei, M. and Kumar, S., 2007, “MEGA 4: Molecular evolutionary genetics analysis (MEGA) software version 4.0.,” Molecular Biology and Evolution, Vol. 24, pp. 1596-1599.

[23] Thompson, J.D., Higgins, D.G. and Gibson, T.J., 1994, “CLUST W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Research, Vol. 22, No. 22, pp. 4673-4680.

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