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2012 Vol.49, Issue 4 Preview Page

Research Paper

Identification of Indigenous Acidophilic Bacteria by Polymerase Chain Reaction and 16S rRNA Sequences

중합효소연쇄반응과 16S rRNA 염기서열을 이용한 토착호산성박테리아의 동정

김봉주·위대웅·백근식·성치남·최낙철·박천영

Bong-Ju Kim, Dae-Woong Wi, Keun-Sik Baik, Chi-Nam Seong, Nag-Chol Choi and Cheon-Young Park

31 August 2012. pp. 507-520
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Abstract
Polymerase chain reaction and 16S rRNA sequence were investigated to identify the indigenous acidophilic bacteria from the Goseong and Yeonhwa in Korea, and the Japanese bacteria in Japan. In comparison sequences with the type strain of Acithiobacillus ferrooxidans(ATCC 23270), the similarity of Goseong and Yeonhwa bacteria were obtained the range of 96.99-97.79% and 97.59-97.90%, respectively. By comparison sequences with type strain both A. ferrooxidans and A. caldus(ATCC 51756) the similarity were founded range of 97.26-97.97% and 93.84% in the Japanese bacteria, respectively. The similarity of these bacteria were found 98.5% with the type strain of Acithiobacillus ferrooxidans by using DGGE in the cluster analysis. When these bacteria were incubated in growth-medium with and without sulfur, the pH value were rapidly decreased more containing ferrous iron medium than medium with sulfur.
Keywords
Indigenous acidophilic bacteria
Polymerase chain reaction
16S rRNA sequence
Type strain
Acidithiobacillus ferrooxidans
고성, 연화 및 일본 토착호산성박테리아를 동정하기 위하여 중합효소연쇄반응과 16S rRNA 염기서열 분석을 수행하였다. 3개 지역 시료에서 얻어진 16S rRNA 염기서열을 표준균주인 Acidithiobacillus ferrooxidans (ATCC 23270)와 pairwise 비교 정렬(alignment sequence) 방법으로 유사도를 비교하였다. 표준균주와 유사도를 비교한 결과, 고성 및 연화 박테리아의 염기서열은 유사도가 각각 96.99-97.79%와 97.59-97.90%로 나타났다. 일본 박테리아는 표준균주와의 유사도가 97.26-97.97%로 나타났고 A. caldus 표준균주와는 93.84%의 유사도를 보였다. 변성구배 겔 전기영동으로 3개 지역 박테리아들에 대한 군집분석을 수행한 결과 고성, 연화 및 일본 박테리아는 모두는 Acidithiobacillus ferrooxidans와 98.5%의 유사도를 보였다. 이들 박테리아들을 이용하여 성장-실험을 수행한 결과 원소 황을 포함하는 배양액에서 보다 ferrous iron을 포함된 배양액에서 더 빠르게 pH가 감소하였다.
키워드
토착호산성박테리아
중합효소연쇄반응
16S rRNA 염기서열
표준균주
Acidithiobacillus ferrooxidans
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Information
  • Publisher :The Korean Society of Mineral and Energy Resources Engineers
  • Publisher(Ko) :한국자원공학회
  • Journal Title :Journal of the Korean Society for Geosystem Engineering
  • Journal Title(Ko) :한국지구시스템공학회지
  • Volume : 49
  • No :4
  • Pages :507-520
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