Geomicrobiological Effects on Arsenic Behavior in Anaerobic Sediment from Abandoned Gold Mine Area

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2006 Vol.43, Issue 5 Preview Page Next Page

Geomicrobiological Effects on Arsenic Behavior in Anaerobic Sediment from Abandoned Gold Mine Area 폐 금광산 주변 혐기성 퇴적물에서 비소 거동에 미치는 지구미생물학적 영향: 이종운, 이상우, 김경웅, 이진수, 전효택
Jong-Un Lee, Sang-Woo Lee, Kyoung-Woong Kim, Jin-Soo Lee and Hyo-Taek Chon

31 October 2006. pp. 448-457

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Abstract

Indigenous bacterial mediation of As geochemistry in As-contaminated sediment from an abandoned Au-Ag mine area was investigated after biostimulation with glucose as a carbon source under anaerobic condition. According to the result of sequential extraction analysis, arsenic mainly existed as Fe-associated and residual fractions in the sediment. After 14-day incubation, the indigenous bacteria increased the amount of dissolved Fe2+ with time. However, dissolved As diminished with time likely due to the production of As sulfide(s) and subsequent precipitation, which resulted from bacterial reduction of SO42-. The results implied that bacterial in-situ stabilization of As in subsurface has a potential to be practically applied.

Keywords

Bacteria

Arsenic

Geomicrobiology

Reduction

Sediment

In-situ stabilization

폐 금은광산 주변의 비소로 오염된 퇴적물을 대상으로 하여 혐기성 조건에서 탄소원으로 포도당을 주입한 후 비소 지구화학에 미치는 토착 박테리아의 영향을 조사하였다. 연속추출 결과에 의하면 퇴적물 내 비소는 주로 철과 결합하거나 잔류상으로 존재하는 것으로 나타났다. 14일에 걸친 반응 결과, 토착 박테리아는 용존 Fe2+ 함량을 증가시켰다. 그러나 용존 비소의 함량은 감소하였으며 이는 박테리아에 의한 황산이온 환원에 의해 비소가 황화물을 형성하며 침전하였기 때문으로 판단되었다. 이러한 결과는 박테리아에 의한 비소의 지하 심부 현장 고정화 기법이 실제적으로 적용 가능할 수 있음을 나타낸다.

키워드

박테리아

비소

지구미생물학

환원

퇴적물

현장 고정화

References

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이종운, 이상우, 김경웅, 2001, “호기성환경에서 비소의 지구화학적 거동에 미치는 미생물의 영향 및 오염 복구에의 적용 가능성”, 자원환경지질, 제34권, pp. 345-354.
안주성, 김주용, 전철민, 문희수, 2003, “풍화광미 내 고상 비소의 광물학적․화학적 특성 및 용출가능성 평가”, 자원환경지질, 제36권, pp. 27-38.
중앙일보, 2006, 9월 5일 중앙일보 사회면.
하원경, 이종운, 정명채, 2006, “우라늄의 지구미생물학적환원성 고정화와 장기적 안정성 연구”, 한국지구시스템 공학회지, 제43권, pp. 331-338.
Aggett, J. and Kriegman, M.R., 1988, “The Extent of Formation of Arsenic(III) in Sediment Interstitial Waters in Lake Ohakuri”, Water Res., Vol. 22, pp. 407-411. Bagla, P. and Kaiser, J., 1996, “India’s Spreading Health Crisis Draws Global Arsenic Experts”, Science, Vol. 274, pp. 174-175.
Brannon, J.M. and Patrick Jr., W.H., 1987, “Fixation, Transformation, and Mobilization of Arsenic in Sediments”, Environ. Sci. Technol., Vol. 21, pp. 450-459.
Brown, D.A., Kamineni, D.C., Sawicki, J.A., and Beveridge, T.J., 1994, “Minerals Associated with Biofilms Occurring on Exposed Rock in a Granitic Underground Research Laboratory”, Appl. Environ. Microbiol., Vol. 60, pp. 3182-3191.
Brown, D.A., Sherriff, B.L., Sawicki, J.A., and Sparling, R., 1999, “Precipitation of Iron Minerals by a Natural Microbial Consortium”, Geochim. Cosmochim. Acta, Vol. 63, pp. 2163-2169.
Cervantes, C., Ji, G., Ramirez, J.L., and Silver, S., 1994, “Resistance to Arsenic Compounds in Microorganisms”, FEMS Microbiol. Rev., Vol. 15, pp. 355-367.
Ilyaletdinov, A.N. and Abdrashitova, S.A., 1981, “Autotrophic Oxidation of Arsenic by a Culture of Pseudomonas arsenitoxidans”, Mikrobiologiya, Vol. 50, pp. 197-204.
Ji, G. and Silver, S., 1995, “Bacterial Resistance Mechanisms for Heavy Metals of Environmental Concern”, J. Ind. Microbiol., Vol. 14, pp. 61-75.
Le, X.C., Yalcin, S. and Ma, M., 2000, “Speciation of Submicrogram per Liter Levels of Arsenic in Water: On-site Species Separation Integrated with Sample Collection”, Environ. Sci. Technol. Vol. 4, pp. 2342-2347.
Lee, J.-U., Lee, S.-W., Kim, K.-W., and Yoon, C.-H., 2005, “The Effects of Different Carbon Sources on Microbial Mediation of Arsenic in Arsenic-contaminated Sediment”, Environ. Geochem. Hlth., Vol. 27, pp. 159-168.
Lee, S.E., Lee, J.-U., Lee, J.S., and Chon, H.T., 2006, “Effects of Indigenous Bacteria on Cr(VI) Reduction in Cr-contaminated Sediment with Industrial Wastes”, J. Geochem. Explor., Vol. 88, pp. 41-44.
Lovley, D.R., 1993, “Dissimilatory Metal Reduction”, Annu. Rev. Microbiol., Vol. 47, pp. 263-290.
Lovley, D.R., Phillips, E.J., and Lonergan, D.J., 1991, “Enzymatic Versus Nonenzymatic Mechanisms for Fe(III) Reduction in Aquatic Sediments”, Environ. Sci. Technol., Vol. 25, pp. 1062-1067.
Malasarn, D., Saltikov, C.W., Campbell, K.M., Santini, J.M., Hering, J.G., and Newman, D.K., 2004, “arrA is a Reliable Marker for As(V) Respiration”, Science, Vol. 306, p. 455.
Masscheleyn, P., Delaune, R., and Patrick Jr., W.H., 1991, “Effect of Redox Potential and pH on Arsenic Speciation and Solubility in a Contaminated Soil”, Environ. Sci. Technol., Vol. 25, pp. 1414-1419.
McLean, J.S., Lee, J.-U., and Beveridge, T.J., 2002, “Interactions of Bacteria and Environmental Metals, Fine-grained Mineral Development, and Bioremediation Strategies”, In: Huang, P.M., Bollag, J.-M., and Senesi, N. (Eds), Interactions between Soil Particles and Microorganisms: Impact on the Terrestrial Ecosystem. John Wiley and Sons, Chichester, England, pp. 227-261.
Mok, W.M. and Wai, C.M., 1994, “Mobilization of Arsenic in Contaminated River Waters”, In: Nriagu, J.O. (Ed), Arsenic in the environment, Wiley, New York, pp. 99-117.
Newman, D.K., Kennedy, E.K., Coates, J.D., Ahmann, D., Ellis, D.J., Lovley, D.R., and Morel, F.M.M., 1997a, “Dissimilatory Arsenate and Sulfate Reduction in Desulfotomaculum auripigmentum sp. nov.”, Arch. Microbiol., Vol. 168, pp. 380-388.
Newman, D.K., Beveridge, T.J., and Morel, F.M.M., 1997b, “Precipitation of Arsenic Trisulfide by Desulfotomaculum auripigmentum”, Appl. Environ. Microbiol., Vol. 63, pp. 2022-2028.
Newman, D.K., Ahmann, D., and Morel, F.M.M., 1998, “A Brief Review of Microbial Arsenate Respiration”, Geomicrobiol. J., Vol. 15, pp. 255-268.
Oremland, R.S. and Stolz, J.F., 2003, “The Ecology of Arsenic”, Science, Vol. 300, pp. 939-944.
Park, J.M., Lee, J.S., Lee, J.-U., Chon, H.T., and Jung, M.C., 2006, “Microbial Effects on Geochemical Behavior of Arsenic in As-contaminated Sediments”, J. Geochem. Explor., Vol. 88, pp. 134-138.
Pierce, M.L. and Moore, C.B., 1982, “Adsorption of Arsenite and Arsenate on Amorphous Iron Hydroxide”, Water Res., Vol. 16, pp. 1247-1253.
Rosen, B.P., Silver, S., Gladysheva, T.B., Ji, G., Oden, K.L., Jagannathan, S., Shi, W., Chen, Y., and Wu, J., 1994, “The Arsenite Oxyanion-translocating ATPase: Bioenergetics, Functions, and Regulation”, In: Torriani- Gorini, A., Yagil, E., and Silver, S. (Eds), Phosphate in microorganisms, ASM Press, Washington, D.C. pp. 97-107.
Salmassi, T.M., Venkateswaren, K., Satomi, M., Nealson, K.H., Newman, D.K., and Hering, J.G., 2002, “Oxidation of Arsenite by Agrobacterium albertimagni, AOL15, sp. nov., Isolated from Hot Creek, California”, Geomicrobiol. J., Vol. 19, pp. 53-66.
Santini, J.M., Sly, L.I., Schnagl, R.D., and Macy, J.M., 2000, “A New Chemolithoautotrophic Arsenite-oxidizing Bacterium Isolated from a Gold Mine: Phylogenetic, Physiological, and Preliminary Biochemical Studies”, Appl. Environ. Microbiol., Vol. 66, pp. 92-97.
Spliethoff, H., Mason, R.P., and Hemond, H.F., 1995, “Interannual Variability in the Speciation and Mobility of Arsenic in a Dimictic Lake”, Environ. Sci. Technol., Vol. 29, pp. 2157-2161.
Stookey, L.L., 1970, “Ferrozine - A New Spectrophotometric Reagent for Iron”, Anal. Chem., Vol. 42, pp. 779-781.
US DOE, 2006, Internet reference, http://www.lbl.gov/NABIR.
US EPA, 2006, Internet reference, http://www.epa.gov/waterscience/cs/vol1/appdx_d.pdf.
Woolson, E.A., Axley, J.H., and Hearney, P.C., 1973, “The Chemistry and Phytotoxicity of Arsenic in Soils: II. Effects of Time and Phosphorous”, Soil. Sci. Soc. Am. Proc., Vol. 37, pp. 254-259

Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society for Geosystem Engineering
Journal Title(Ko) :한국지구시스템공학회지
Volume : 43
No :5
Pages :448-457

[1] 이성은, 이종운, 이진수, 전효택, 2005, “혐기성 퇴적물에서 토착미생물에 의한 6가 크롬의 환원”, 한국지구시스템공학회지, 제42권, pp. 618-624.

[2] 이종운, 이상우, 김경웅, 2001, “호기성환경에서 비소의 지구화학적 거동에 미치는 미생물의 영향 및 오염 복구에의 적용 가능성”, 자원환경지질, 제34권, pp. 345-354.

[3] 안주성, 김주용, 전철민, 문희수, 2003, “풍화광미 내 고상 비소의 광물학적․화학적 특성 및 용출가능성 평가”, 자원환경지질, 제36권, pp. 27-38.

[4] 중앙일보, 2006, 9월 5일 중앙일보 사회면.

[5] 하원경, 이종운, 정명채, 2006, “우라늄의 지구미생물학적환원성 고정화와 장기적 안정성 연구”, 한국지구시스템 공학회지, 제43권, pp. 331-338.

[6] Aggett, J. and Kriegman, M.R., 1988, “The Extent of Formation of Arsenic(III) in Sediment Interstitial Waters in Lake Ohakuri”, Water Res., Vol. 22, pp. 407-411. Bagla, P. and Kaiser, J., 1996, “India’s Spreading Health Crisis Draws Global Arsenic Experts”, Science, Vol. 274, pp. 174-175.

[7] Brannon, J.M. and Patrick Jr., W.H., 1987, “Fixation, Transformation, and Mobilization of Arsenic in Sediments”, Environ. Sci. Technol., Vol. 21, pp. 450-459.

[8] Brown, D.A., Kamineni, D.C., Sawicki, J.A., and Beveridge, T.J., 1994, “Minerals Associated with Biofilms Occurring on Exposed Rock in a Granitic Underground Research Laboratory”, Appl. Environ. Microbiol., Vol. 60, pp. 3182-3191.

[9] Brown, D.A., Sherriff, B.L., Sawicki, J.A., and Sparling, R., 1999, “Precipitation of Iron Minerals by a Natural Microbial Consortium”, Geochim. Cosmochim. Acta, Vol. 63, pp. 2163-2169.

[10] Cervantes, C., Ji, G., Ramirez, J.L., and Silver, S., 1994, “Resistance to Arsenic Compounds in Microorganisms”, FEMS Microbiol. Rev., Vol. 15, pp. 355-367.

[11] Ilyaletdinov, A.N. and Abdrashitova, S.A., 1981, “Autotrophic Oxidation of Arsenic by a Culture of Pseudomonas arsenitoxidans”, Mikrobiologiya, Vol. 50, pp. 197-204.

[12] Ji, G. and Silver, S., 1995, “Bacterial Resistance Mechanisms for Heavy Metals of Environmental Concern”, J. Ind. Microbiol., Vol. 14, pp. 61-75.

[13] Le, X.C., Yalcin, S. and Ma, M., 2000, “Speciation of Submicrogram per Liter Levels of Arsenic in Water: On-site Species Separation Integrated with Sample Collection”, Environ. Sci. Technol. Vol. 4, pp. 2342-2347.

[14] Lee, J.-U., Lee, S.-W., Kim, K.-W., and Yoon, C.-H., 2005, “The Effects of Different Carbon Sources on Microbial Mediation of Arsenic in Arsenic-contaminated Sediment”, Environ. Geochem. Hlth., Vol. 27, pp. 159-168.

[15] Lee, S.E., Lee, J.-U., Lee, J.S., and Chon, H.T., 2006, “Effects of Indigenous Bacteria on Cr(VI) Reduction in Cr-contaminated Sediment with Industrial Wastes”, J. Geochem. Explor., Vol. 88, pp. 41-44.

[16] Lovley, D.R., 1993, “Dissimilatory Metal Reduction”, Annu. Rev. Microbiol., Vol. 47, pp. 263-290.

[17] Lovley, D.R., Phillips, E.J., and Lonergan, D.J., 1991, “Enzymatic Versus Nonenzymatic Mechanisms for Fe(III) Reduction in Aquatic Sediments”, Environ. Sci. Technol., Vol. 25, pp. 1062-1067.

[18] Malasarn, D., Saltikov, C.W., Campbell, K.M., Santini, J.M., Hering, J.G., and Newman, D.K., 2004, “arrA is a Reliable Marker for As(V) Respiration”, Science, Vol. 306, p. 455.

[19] Masscheleyn, P., Delaune, R., and Patrick Jr., W.H., 1991, “Effect of Redox Potential and pH on Arsenic Speciation and Solubility in a Contaminated Soil”, Environ. Sci. Technol., Vol. 25, pp. 1414-1419.

[20] McLean, J.S., Lee, J.-U., and Beveridge, T.J., 2002, “Interactions of Bacteria and Environmental Metals, Fine-grained Mineral Development, and Bioremediation Strategies”, In: Huang, P.M., Bollag, J.-M., and Senesi, N. (Eds), Interactions between Soil Particles and Microorganisms: Impact on the Terrestrial Ecosystem. John Wiley and Sons, Chichester, England, pp. 227-261.

[21] Mok, W.M. and Wai, C.M., 1994, “Mobilization of Arsenic in Contaminated River Waters”, In: Nriagu, J.O. (Ed), Arsenic in the environment, Wiley, New York, pp. 99-117.

[22] Newman, D.K., Kennedy, E.K., Coates, J.D., Ahmann, D., Ellis, D.J., Lovley, D.R., and Morel, F.M.M., 1997a, “Dissimilatory Arsenate and Sulfate Reduction in Desulfotomaculum auripigmentum sp. nov.”, Arch. Microbiol., Vol. 168, pp. 380-388.

[23] Newman, D.K., Beveridge, T.J., and Morel, F.M.M., 1997b, “Precipitation of Arsenic Trisulfide by Desulfotomaculum auripigmentum”, Appl. Environ. Microbiol., Vol. 63, pp. 2022-2028.

[24] Newman, D.K., Ahmann, D., and Morel, F.M.M., 1998, “A Brief Review of Microbial Arsenate Respiration”, Geomicrobiol. J., Vol. 15, pp. 255-268.

[25] Oremland, R.S. and Stolz, J.F., 2003, “The Ecology of Arsenic”, Science, Vol. 300, pp. 939-944.

[26] Park, J.M., Lee, J.S., Lee, J.-U., Chon, H.T., and Jung, M.C., 2006, “Microbial Effects on Geochemical Behavior of Arsenic in As-contaminated Sediments”, J. Geochem. Explor., Vol. 88, pp. 134-138.

[27] Pierce, M.L. and Moore, C.B., 1982, “Adsorption of Arsenite and Arsenate on Amorphous Iron Hydroxide”, Water Res., Vol. 16, pp. 1247-1253.

[28] Rosen, B.P., Silver, S., Gladysheva, T.B., Ji, G., Oden, K.L., Jagannathan, S., Shi, W., Chen, Y., and Wu, J., 1994, “The Arsenite Oxyanion-translocating ATPase: Bioenergetics, Functions, and Regulation”, In: Torriani- Gorini, A., Yagil, E., and Silver, S. (Eds), Phosphate in microorganisms, ASM Press, Washington, D.C. pp. 97-107.

[29] Salmassi, T.M., Venkateswaren, K., Satomi, M., Nealson, K.H., Newman, D.K., and Hering, J.G., 2002, “Oxidation of Arsenite by Agrobacterium albertimagni, AOL15, sp. nov., Isolated from Hot Creek, California”, Geomicrobiol. J., Vol. 19, pp. 53-66.

[30] Santini, J.M., Sly, L.I., Schnagl, R.D., and Macy, J.M., 2000, “A New Chemolithoautotrophic Arsenite-oxidizing Bacterium Isolated from a Gold Mine: Phylogenetic, Physiological, and Preliminary Biochemical Studies”, Appl. Environ. Microbiol., Vol. 66, pp. 92-97.

[31] Spliethoff, H., Mason, R.P., and Hemond, H.F., 1995, “Interannual Variability in the Speciation and Mobility of Arsenic in a Dimictic Lake”, Environ. Sci. Technol., Vol. 29, pp. 2157-2161.

[32] Stookey, L.L., 1970, “Ferrozine - A New Spectrophotometric Reagent for Iron”, Anal. Chem., Vol. 42, pp. 779-781.

[33] US DOE, 2006, Internet reference, http://www.lbl.gov/NABIR.

[34] US EPA, 2006, Internet reference, http://www.epa.gov/waterscience/cs/vol1/appdx_d.pdf.

[35] Woolson, E.A., Axley, J.H., and Hearney, P.C., 1973, “The Chemistry and Phytotoxicity of Arsenic in Soils: II. Effects of Time and Phosphorous”, Soil. Sci. Soc. Am. Proc., Vol. 37, pp. 254-259

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