Heavy Metal Stabilization in Deep Agricultural Soils Using Sulfate-Reducing Bacteria

Yejin Choi; Eui-Jeong Hwang; Hyunjoon Hwang; Hyeop-Jo Han; Jong-Un Lee

doi:10.32390/ksmer.2023.60.3.151

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2023 Vol.60, Issue 3 Next Page

Research Paper

Heavy Metal Stabilization in Deep Agricultural Soils Using Sulfate-Reducing Bacteria 황산염환원균을 이용한 농경지 심부 토양 내 중금속 안정화 연구

30 June 2023. pp. 151-160

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Abstract

Heavy metal stabilization in soil was investigated using sulfate-reducing bacteria (SRB). Samples were collected from three agricultural soils near abandoned mines, and a nine-week batch experiment was performed following the classification of soils as ① non-sterile soil with SO₄^2- (SO4), ② sterile soil with SRB (SRBo), and ③ non-sterile soil with SRB and SO₄^2- (SS). Heavy metals were extracted using 0.1 N HCl or Mehlich, and the stabilization efficiency was evaluated by comparing the results to the control. Pb, Zn, Cu, and Cd (4–55%) had higher stabilization efficiency than the control in Mehlich extraction, while Pb (2–41%) had higher stabilization efficiency than the control in 0.1 N HCl extraction. The SS samples demonstrated a high stabilization efficiency (17–55%), whereas the SRBo samples (2–37%) showed a low efficiency. Dissolved As decreased in the supernatant of the SO4 and SS samples, especially in the high-As soil. These results indicate that incorporating SRB and SO₄^2- into soil contaminated with heavy metals can effectively decrease the mobility of As and heavy metals.

Keywords

sulfate-reducing bacteria

soil

heavy metals

arsenic

stabilization

황산염환원균(SRB)을 이용하여 심부 토양 내 용존 중금속을 안정화시키는 연구를 수행하였다. 폐광산 주변 농경지 3 곳에서 토양을 채취하여 ① 비멸균 토양에 SO₄^2-를 공급한 경우(SO4), ② 멸균 토양에 SRB를 접종한 경우(SRBo), ③ 비멸균 토양에 SRB와 SO₄^2-를 공급한 경우(SS)로 나누어 9주에 걸친 배치 실험을 수행하였다. 1, 4, 7, 9주에 0.1 N HCl 및 Mehlich 단일용출법으로 토양을 용출한 후 비교시료에 대한 안정화 효율을 평가하였다. Mehlich 용출을 통해서는 Pb, Zn, Cu, Cd(4~55%), 0.1 N HCl 용출 결과, Pb(2~41%)가 비교시료에 비하여 높은 안정화 효율을 보였다. 또한 SS 시료(17~55%)에서 높은 안정화 효율을 보인 반면, SRBo 시료(2~37%)에서는 낮은 효율을 보였다. 토양 상등액을 채취하여 분석한 결과, As로 심각하게 오염된 토양의 SO4와 SS 시료에서 As 용출량이 감소하였다. 이는 중금속 오염 심부 토양에 SRB 및 적절한 SO₄^2-를 공급하면 As 및 중금속의 이동도를 효과적으로 감소시킬 수 있음을 나타낸다.

키워드

황산염환원균

토양

중금속

비소

안정화

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Information

Publisher :The Korean Society of Mineral and Energy Resources Engineers
Publisher(Ko) :한국자원공학회
Journal Title :Journal of the Korean Society of Mineral and Energy Resources Engineers
Journal Title(Ko) :한국자원공학회지
Volume : 60
No :3
Pages :151-160
Received Date : 2023-05-10
Revised Date : 2023-06-12
Accepted Date : 2023-06-26
DOI :https://doi.org/10.32390/ksmer.2023.60.3.151

Journal of the Korean Society of Mineral and Energy Resources Engineers ISSN:2288-0291(Print) 2288-2790(Online) 한국자원공학회지

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